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Lophatherum gracile extract, monomer thereof and application of lophatherum gracile extract to preparation of composition for preventing or treating cancers

A technology of pale bamboo leaf and composition, which is applied in the field of pale bamboo leaf extract and its monomers and the preparation of compositions for preventing or treating cancer, so as to achieve the effect of inhibiting the growth of cancer cells

Inactive Publication Date: 2020-12-15
SAGITTARIUS LIFE SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bamboo leaves are believed to have antipyretic, diuretic, and antibacterial effects. However, whether it has anti-cancer effects is unknown

Method used

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  • Lophatherum gracile extract, monomer thereof and application of lophatherum gracile extract to preparation of composition for preventing or treating cancers
  • Lophatherum gracile extract, monomer thereof and application of lophatherum gracile extract to preparation of composition for preventing or treating cancers
  • Lophatherum gracile extract, monomer thereof and application of lophatherum gracile extract to preparation of composition for preventing or treating cancers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1, preparation of light bamboo leaf extract

[0042] Using an alcoholic solvent to extract the leaves of Palisophyllum edulis to obtain a leaf extract of Phyllostachys edulis (LGCH). Then use the macroporous resin column, ethanol-water gradient elution to separate the light bamboo leaf extract into light bamboo leaf active fractions (LGCH1-1, LGCH1-2, LGCH2, LGCH3, LGCH4 and LGCH5), and make light bamboo leaf active Differentiate the lyophilized product, and use UPLC to detect and analyze its monomer components. Wherein, the alcohol solvent includes but not limited to methanol, ethanol or propanol.

[0043] cell experiment

Embodiment 2

[0044] Example 2. Anticancer Effect Test of Leaf Extract of Bamboo

[0045] Cell treatment method: take the light bamboo leaf extract (LGCH) described in Example 1, use 100% DMSO to prepare it into a 50 mg / mL light bamboo leaf extract stock solution, and then use 5% fetal bovine serum in the culture medium to prepare it Diluted into different concentration (2000, 1500, 1000, 500, 250, 125, 62.5, 31.25 μg / mL) of the diluent of the extract of Paeonia basilica. Take 20 μl of the diluents of the above-mentioned dilute bamboo leaf extract of different concentrations, add them to the cells respectively (final volume: 200 μl / well), and store at 37°C, 5% CO 2 After 48 hours of conditioned culture, cell viability assays were performed.

[0046] Cell viability analysis (MTS assay): After the above-mentioned differently treated cells were cultured for 48 hours, the medium containing the diluent of the extract of Pachyphyllum mellifera was removed, and then 100 μl of MTS reagent (using 5...

Embodiment 3

[0050] Embodiment 3, the anticancer effect test of the active fractionation of the leaves of the light bamboo

[0051] Cell treatment method: get the active subdivision of bamboo leaves described in Example 1, use secondary water (for LGCH1-1, LGCH1-2 and LGCH2) or 100% DMSO (for LGCH3, LGCH4 and LGCH5) to dilute bamboo leaves The active region substance is formulated into 50 mg / mL of the active region substance stock solution of the leaf of the bamboo, and then it is diluted into different concentrations (2000, 1500, 1000, 500, 250, 125, 62.5, 31.25 μg) with the medium containing 5% fetal bovine serum / mL) of the dilution of the active fraction of bamboo leaves. Take 20 μl of the above-mentioned different concentrations of the active fractions of the leaves of Pachyrhiza edulis, add them to the cells respectively (final volume 200 μl / well), and incubate at 37°C, 5% CO 2 After 48 hours of conditioned culture, cell viability assays were performed.

[0052] Cell viability anal...

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Abstract

The invention relates to the field of plant extraction, in particular to a lophatherum gracile extract, a monomer thereof and an application of the lophatherum gracile extract to preparation of a composition for preventing or treating cancers. The invention provides the application of the lophatherum gracile extract to preparation of the composition for treating cancer, an application of a lophatherum gracile active monomer to preparation of a composition for treating cancer, and a composition for treating or preventing cancer. Active ingredients and the monomer of the lophatherum gracile extract comprise orientin, vitexin, isoorientin, isovitexin, swertiajaponin, schaftoside or isoschaftoside.

Description

technical field [0001] The invention relates to the field of plant extraction, in particular to the use of an extract from leaves of phlox and its monomers for preparing a composition for preventing or treating cancer. Background technique [0002] Cancer refers to the abnormal division and proliferation of cells, causing damage to adjacent tissues and organs, and even metastasis to other parts of the body, so it is also called malignant tumor. Cancers are usually named based on the primary site, and cancers that have metastasized from the primary site to other sites are called metastatic cancers from the primary site. [0003] At present, there are more than one hundred kinds of cancers known in the human body, which is an important issue that every country must face. Therefore, effective active ingredients that can be used in the treatment of cancer and are helpful for the development of new drugs have also become the targets actively sought by the academic circles and th...

Claims

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Application Information

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IPC IPC(8): A61K36/899A61K31/352A61P35/00
CPCA61K36/899A61K31/352A61P35/00A61K2300/00
Inventor 陈乐维施明吉
Owner SAGITTARIUS LIFE SCI
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