Biological extraction method of resveratrol

A resveratrol extraction technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of long production cycle, low extraction rate, low purity, etc.

Active Publication Date: 2020-12-15
山西纳安健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a biological extraction method of resver

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A kind of biological extraction method of resveratrol, concrete steps are as follows:

[0040] (1) Wash and pulverize fresh Polygonum cuspidatum root, add it to deionized water, stir and beat, homogenize under high pressure, and use lysozyme for the first enzymatic hydrolysis to obtain Polygonum cuspidatum root slurry;

[0041] (2) Then the Polygonum cuspidatum root slurry is made into a fermentation medium, inoculated with acid-producing bacteria, fermented for 15 hours, added xylanase and β-glucanase, carried out the second enzymolysis, electron beam irradiation for 8 minutes, Continue to ferment for 10 hours, and centrifuge to obtain a fermented liquid;

[0042] (3) Finally, the fermented liquid is extracted with magnetic ionic liquid containing carbon nanotubes to obtain resveratrol;

[0043] Wherein, the magnetic ionic liquid is made from 4-hydroxy-2,2,6,6-tetramethylpiperidine oxide, 1,1,3,3-tetramethylguanidine, and ethyl bromide; The magnetic ionic liquid cont...

Embodiment 2

[0063] A kind of biological extraction method of resveratrol, concrete steps are as follows:

[0064] (1) Wash and pulverize fresh Polygonum cuspidatum root, add it to deionized water, stir and beat, homogenize under high pressure, and use lysozyme for the first enzymatic hydrolysis to obtain Polygonum cuspidatum root slurry;

[0065] (2) Then the Polygonum cuspidatum root slurry is made into a fermentation medium, inoculated with acid-producing bacteria, fermented for 18 hours, added xylanase and β-glucanase, carried out the second enzymolysis, electron beam irradiation for 5 minutes, Continue to ferment for 12 hours and centrifuge to obtain a fermented liquid;

[0066] (3) Finally, the fermented liquid is extracted with magnetic ionic liquid containing carbon nanotubes to obtain resveratrol;

[0067] Wherein, the magnetic ionic liquid is made from 4-hydroxy-2,2,6,6-tetramethylpiperidine oxide, 1,1,3,3-tetramethylguanidine, and ethyl bromide; The magnetic ionic liquid conta...

Embodiment 3

[0087] A kind of biological extraction method of resveratrol, concrete steps are as follows:

[0088](1) Wash and pulverize fresh Polygonum cuspidatum root, add it to deionized water, stir and beat, homogenize under high pressure, and use lysozyme for the first enzymatic hydrolysis to obtain Polygonum cuspidatum root slurry;

[0089] (2) Then the Polygonum cuspidatum root slurry is made into a fermentation medium, inoculated with acid-producing bacteria, fermented for 16 hours, added xylanase and β-glucanase, carried out the second enzymolysis, and electron beam irradiation for 7 minutes, Continue to ferment for 11 hours and centrifuge to obtain a fermented liquid;

[0090] (3) Finally, the fermented liquid is extracted with magnetic ionic liquid containing carbon nanotubes to obtain resveratrol;

[0091] Wherein, the magnetic ionic liquid is made from 4-hydroxy-2,2,6,6-tetramethylpiperidine oxide, 1,1,3,3-tetramethylguanidine, and ethyl bromide; The magnetic ionic liquid co...

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Abstract

The invention provides a biological extraction method of resveratrol. According to the method, fresh polygonum cuspidatum roots are used as raw materials for biological extraction of resveratrol, thecontent of polydatin and resveratrol in the raw materials is high, and a new thought is provided for large-scale preparation of resveratrol. Specifically, the preparation method comprises the following steps of cleaning and crushing fresh polygonum cuspidatum roots, adding the crushed polygonum cuspidatum roots into deionized water, stirring, pulping, carrying out high-pressure homogenization, andcarrying out enzymolysis for the first time by utilizing lysozyme to obtain polygonum cuspidatum root slurry; preparing a fermentation medium from the polygonum cuspidatum root slurry, inoculating acid-producing bacteria, performing fermenting for 15-18 hours, adding xylanase and beta-glucanase, carrying out secondary enzymolysis, carrying out electron beam irradiation for 5-8 minutes, continuingto ferment for 10-12 hours, and performing centrifuging to obtain fermentation liquor; and finally, extracting fermentation liquor by using magnetic ionic liquid containing carbon nanotubes to obtainresveratrol. According to the method, the production period is short, the extraction rate is high, and the purity of the obtained resveratrol is high.

Description

technical field [0001] The invention relates to the technical field of extraction of natural medicines, in particular to a biological extraction method of resveratrol. Background technique [0002] The chemical name of resveratrol is 3,5,4'-stilbene triol (trihydroxyl 1,2-diphenylethylene), which has two structures, cis and trans, and the trans structure is more stable. It is a biological Strong natural polyphenols. Studies have shown that Res can inhibit tumors, anti-oxidation, anti-free radicals, anti-thrombosis, anti-allergy, anti-atherosclerosis, and has preventive effects on coronary heart disease, ischemic heart disease, hyperlipidemia, etc. Resveratrol has It is listed as one of the most promising anti-cardiovascular and anti-cancer drugs, and has been widely used in food additives, health products, cosmetics and other industries. [0003] Resveratrol exists in grape skins, roots and rhizomes of Polygonum cuspidatum, red coats and kernels of leguminous peanuts, amon...

Claims

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Application Information

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IPC IPC(8): C12P7/22C07C37/72C07C39/21C12R1/02C12R1/07C12R1/145
CPCC12P7/22C07C37/004C07C37/72C07C39/21
Inventor 廖国庆
Owner 山西纳安健康科技有限公司
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