Rapid, simple and convenient pretreatment detection method for pesticide residues in fruits and vegetables

A pesticide residue and detection method technology, applied in the field of pesticide technology detection, can solve the problems of reduced accuracy of detection results, large impact of impurities, consumption of reagents and consumables, etc., to reduce the loss of pesticides, less harm to operators, and reduce degradation Effect

Pending Publication Date: 2020-12-15
鲍忠赞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The above method cannot fully separate the pesticides, and the presence of impurities has a great impact, which affects the detection efficiency, consumes too much reagents and consumables, and reduces the accuracy of the detection results.

Method used

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  • Rapid, simple and convenient pretreatment detection method for pesticide residues in fruits and vegetables
  • Rapid, simple and convenient pretreatment detection method for pesticide residues in fruits and vegetables

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The invention provides a fast and simple pretreatment detection method for pesticide residues in fruits and vegetables, and the specific operation steps are as follows:

[0027] Step 1: Clean the outer surface of the sample, collect freshly picked fruits and vegetables, use a hot air blower to blow the surface, remove the adhered dust and water vapor, and set aside;

[0028] Step 2: The sample is crushed, and the above-mentioned raw materials are chopped into small pieces as the sample raw materials, and 1kg of the raw materials are taken as the test samples, and the biological inhibitor is added to the sample and stirred evenly. The biological inhibitor is set to sodium trizide, and The added volume ratio of sodium trizide to the sample is set as sodium trizide:sample=1:20, and crushed with a high-speed tissue grinder;

[0029] Step 3: Sterilize the sample. Stir the mashed sample quickly to homogenate. During the homogenization process, use X-rays and ultraviolet germi...

Embodiment 2

[0035] The invention provides a fast and simple pretreatment detection method for pesticide residues in fruits and vegetables, and the specific operation steps are as follows:

[0036] Step 1: Clean the outer surface of the sample, collect freshly picked fruits and vegetables, use a hot air blower to blow the surface, remove the adhered dust and water vapor, and set aside;

[0037]Step 2: The sample is crushed, and the above-mentioned raw materials are chopped into small pieces as the sample raw materials, and 1kg of the raw materials are taken as the test samples, and the biological inhibitor is added to the sample and stirred evenly. The biological inhibitor is set to sodium trizide, and The added volume ratio of sodium trizide to the sample is set as sodium trizide:sample=1.3:20, and crushed with a high-speed tissue grinder;

[0038] Step 3: Sterilize the sample, stir the crushed sample quickly to homogenate, use X-ray and ultraviolet germicidal lamp to irradiate the upper ...

Embodiment 3

[0044] The invention provides a fast and simple pretreatment detection method for pesticide residues in fruits and vegetables, and the specific operation steps are as follows:

[0045] Step 1: Clean the outer surface of the sample, collect freshly picked fruits and vegetables, use a hot air blower to blow the surface, remove the adhered dust and water vapor, and set aside;

[0046] Step 2: The sample is crushed, and the above-mentioned raw materials are chopped into small pieces as the sample raw materials, and 1kg of the raw materials are taken as the test samples, and the biological inhibitor is added to the sample and stirred evenly. The biological inhibitor is set to sodium trizide, and The added volume ratio of sodium trizide to the sample is set as sodium trizide:sample=1.8:20, and crushed with a high-speed tissue grinder;

[0047] Step 3: Sterilize the sample, stir the crushed sample quickly to homogenate, use X-ray and ultraviolet germicidal lamp to irradiate the upper...

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Abstract

The invention discloses a rapid, simple and convenient pretreatment detection method for pesticide residues in fruits and vegetables. The method comprises the following steps: cleaning the outer surface of a sample, crushing the sample, sterilizing the sample, centrifugally splitting the sample, passing the sample through an anhydrous magnesium sulfate column and purifying and detecting the sample. The air heater blows the surface of a sample to reduce interference of impurities and water vapor on detection of pesticide residues, the biological inhibitor cooperates with X-rays and the ultraviolet germicidal lamp for sterilization and then cooperates with CuSO4 to deactivate biological factors, degradation of unstable pesticides is reduced, the stability of the pesticide residues is improved, deterioration and damage of the pesticide residues are prevented through ultralow-temperature freezing and water bath pyrolysis. Pesticide residues are further precipitated. An acetonitrile acetatesolution and a buffer mixture are matched with a refrigerated centrifuge for high-speed separation, so that the degradation rate of the pesticide residues is reduced, an acetonitrile phase and a water phase are layered, the working efficiency is improved, a C18 + graphitized carbon black mixture is used for purification, the adsorbability to the pesticide is small, and the recovery rate of the pesticide residues is increased.

Description

technical field [0001] The invention relates to the technical detection field of pesticides, in particular to a fast and simple pretreatment detection method for pesticide residues in fruits and vegetables. Background technique [0002] Pesticide residues are the general term for trace amounts of pesticide precursors, toxic metabolites, degradation products and impurities that remain in organisms, harvests, soil, water and the atmosphere without being decomposed within a period of time after pesticide use, especially pesticide residues in fruits and vegetables The amount is of great significance for continuous research on pest and disease resistance, and it can also reasonably plan the drug use cycle to avoid food poisoning caused by excessive residues. [0003] In the prior art, after the fruits and vegetables are sampled, the samples to be tested are prepared and stored at -20°C to -16°C. Take 25.0g of the samples in a beaker, add 50.0mL of acetonitrile, and homogenize for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34G01N1/28A23L5/20G01N1/38G01N33/02
CPCG01N1/34G01N1/286A23L5/20G01N1/38G01N33/025
Inventor 鲍忠赞张喜悦
Owner 鲍忠赞
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