PTPN2-blocked immune cell and application and preparation thereof

A technology of immune cells and NKT cells, applied in the field of PTPN2-blocking immune cells and their applications and preparations, can solve the problems of solid tumors without good treatment effect, low clinical response rate, off-target, etc., to enhance the killing function and reduce exhaustion. , to avoid toxic effects

A technology of immune cells and NKT cells, applied in the field of PTPN2-blocking immune cells and their applications and preparations, can solve the problems of solid tumors without good treatment effect, low clinical response rate, off-target, etc., to enhance the killing function and reduce exhaustion. , to avoid toxic effects

CN112111457AInactive Publication Date: 2020-12-22GUANGDONG XIANKANGDA BIOTECH CO LTD
1 Cites 0 Cited by

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • PTPN2-blocked immune cell and application and preparation thereof
  • PTPN2-blocked immune cell and application and preparation thereof
  • PTPN2-blocked immune cell and application and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] The preparation method and functional verification of immune cells in this embodiment include the following steps:

[0081] Step 1: Isolation of PBMC from peripheral blood and expansion of T cells

[0082] Mononuclear cells were isolated from donor peripheral blood, density gradient centrifugation was performed using ficol, and T cells were enriched with a T cell sorting kit (CD3 MicroBeads, human-lyophilized, 130-097-043), using conjugated anti-CD3 / anti-CD28 magnetic beads activated culture and expansion of T cells; the medium used TexMACS GMP Medium (MiltenyiBiotec, 170-076-309), containing 10% FBS, 2mM L-glutamine, 100IU / ml rhIL2, all cells were placed Cultured in a constant temperature incubator at 37°C with 5% CO2.

[0083] Step 2: Cell Line Culture

[0084] Cell line expressing GPC3: Huh-7 (human liver cancer cell), purchased from ATCC.

[0085] Cell line not expressing GPC3: A549 (human non-small cell lung cancer cells), purchased from ATCC.

[0086] Cells f...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of biological cells, and particularly relates to a PTPN2-blocked immune cell and application and a preparation thereof. The PTPN2-blocked immune cell expresses a chimeric antigen receptor, the PTPN2 function in the immune cell is blocked, and the immune cell can secrete an anti-PD-1 antibody. The invention provides a method for killing tumors and reversing exhaustion. The PTPN2-blocked function is innovatively combined with CAR and anti-PD-1 immunoregulation, so that the tumor killing activity of the immune cell is improved, and the effect of curing tumors isachieved.

Description

technical field [0001] The present invention relates to the technical field of biological cells, in particular to an immune cell that blocks PTPN2 and its application and preparation, an immune cell that expresses CAR, blocks the function of intracellular PTPN2 protein, and optimizes the expansion ability and killing function of CD8+ T cells. And combined with the PD-1 inhibitory pathway, the immunotherapy response was more pronounced. Background technique [0002] In recent years, tumor immunotherapy has developed rapidly, especially adoptive cell therapy (ACT), which means that immune cells such as T cells and NK cells are isolated from patients, modified and expanded in vitro, and then reintroduced into patients for tumor treatment. method of treatment. In 2013, the immunotherapy of tumors was rated as the first among the "Top Ten Breakthroughs" by Science Impurities. [0003] CAR-T and TCR-T are important components of adoptive cell therapy, especially CAR-T therapy, w...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
22 Dec 2020
Publication
CN112111457A
IPC
C12N5/10; C12N15/867; C12N15/62; C12N15/13; A61K39/00; A61P35/00
CPC
A61K2039/5156; A61P35/00; A61K39/001102; C07K14/7051; C07K16/2818; C07K16/303; C07K16/40; C07K2317/622
Inventors
马丽雅; 贾晓晓