Crohn's disease (CD) related microbial marker set and application thereof

A technology for Crohn's disease and microbes, applied in the field of biomarkers, can solve problems such as persistent non-healing and repeated attacks of patients, and achieve the effects of non-invasive sampling methods, accurate results, and easy transportation

Pending Publication Date: 2020-12-22
康美华大基因技术有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease can be improved after treatment, but most patients will have recurrent attacks and protracted healing, and many patients will also have multiple complications that require surgical treatment

Method used

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  • Crohn's disease (CD) related microbial marker set and application thereof
  • Crohn's disease (CD) related microbial marker set and application thereof
  • Crohn's disease (CD) related microbial marker set and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] 1) Data collection

[0056] Based on a large number of literature surveys on Crohn's disease, the inventor adopted a meta-analysis strategy and collected 3 intestinal flora metagenomic sequencing data sets (denoted as C1 group, C2 group, and C3 group) designed for disease control, including None of the 117 patients with Crohn's disease and 146 healthy controls received antibiotics or invasive medical interventions. As shown in Table 1.

[0057] Table 1 Characteristics of Crohn's disease population data set

[0058]

[0059] 2) Sequence alignment

[0060] After filtering the sequencing data of the data set in Table 1, the inventors used metaphlan2 to compare the sequencing sequences to the human intestinal microbial marker gene set, and constructed the species abundance profiles of 265 microorganisms.

[0061] 3) Clinical association analysis

[0062] In order to measure the impact of different clinical phenotypes on the species spectrum, the inventors used the pe...

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Abstract

The invention discloses a Crohn's disease (CD) related microbial marker set and application thereof in preparation of a CD detection reagent or detection kit. The CD related microbial marker set comprises 34 human intestinal microorganisms screened specifically, has high accuracy when used for diagnosing CD, and can realize early screening of the CD, save time for patients, achieve early treatmentand improve the treatment effects. The invention also discloses application of the CD related microbial marker set in CD detection. Human excrement is taken as a sample, the operation and transportation are convenient, the accuracy of detection results reaches 95.2%, and the sensitivity reaches 91.4%. The invention also discloses the CD detection kit comprising the CD related microbial marker set. The CD detection kit is convenient to use, can comprehensively detect and monitor intestinal flora difference conditions in a disease state, and is used for early discovery of CD patients.

Description

technical field [0001] The invention relates to the technical field of biomarkers, in particular to a combination of Crohn's disease-related microbial markers and its application. Background technique [0002] Crohn's disease (CD) is a chronic gastrointestinal inflammatory disease that, together with ulcerative colitis (UC), is currently the most common inflammatory bowel disease, affecting 7% to 21% of the global population health. While UC often presents as persistent inflammation and ulceration of the innermost lining of the colon and rectum, CD can cause inflammation anywhere in the digestive tract. Clinically, patients with Crohn's disease often present with severe diarrhea, abdominal pain, fatigue, weight loss, rectal bleeding or bloody stools; and even various systemic complications, such as blurred vision, joint pain, rash and other symptoms. The disease can be improved after treatment, but most patients will have recurrent attacks and protracted healing, and many ...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/6869G16B30/10G06N20/00G16H50/20
CPCC12Q1/689C12Q1/6869G16B30/10G06N20/00G16H50/20Y02A90/10
Inventor 杨超
Owner 康美华大基因技术有限公司
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