Application of 6BIO in aspect of heart aging resistance
A kind of heart aging technology, applied in the application field of 6BIO in anti-cardiac aging
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[0032] Application of 6BIO in anti-aging of the heart
[0033] Materials and methods
[0034] 1. Animals and Handling
[0035] We obtained male young (2 months) and aged (18 months) C57BL / 6J mice from the Experimental Animal Center of the Fourth Military Medical University of the Chinese People's Liberation Army. All mice were housed in a specific pathogen-free (SPF) environment with light and temperature control, and had free access to food and water. Our experimental mice were divided into the following four groups, with eight mice in each group:
[0036] (1) Young male rats were intraperitoneally injected with 0.9% sodium chloride (10 ml / kg) every day;
[0037] (2) Aged male mice were intraperitoneally injected with 0.9% sodium chloride (10 ml / kg) every day;
[0038] (3) Treat aging male mice with rapamycin, inject rapamycin (4mg / kg) 10ml / kg saline intraperitoneally every day;
[0039] (4) Treat aged male mice with 6BIO, and inject 6BIO (10mg / kg) 10ml / kg saline intrape...
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