Sargassum fusiforme polysaccharide and extraction method thereof

Abstract

The invention discloses sargassum fusiforme polysaccharide and an extraction method thereof. The extraction method comprises the following steps: crushing and sieving sargassum fusiforme, adding the sargassum fusiforme into an ethanol solution, heating the raw materials for reflux reaction, filtering the product, and drying the filter residues; adding the filter residues into a solvent, uniformlymixing the solvent to obtain a sargassum fusiforme solution, carrying out ultrasonic and water bath extraction treatment, and centrifuging the solution to obtain an extract liquid; carrying out rotaryevaporation and concentration on the extract liquid to obtain a concentrated solution, carrying out precipitation treatment, and centrifuging the liquid to obtain a precipitate, namely, crude polysaccharide; removing protein from the crude polysaccharide by a Sevage method, dialyzing the liquid to remove salt, and freeze-drying the dialyzate to obtain the sargassum fusiforme polysaccharide. According to the extraction method of sargassum fusiforme polysaccharide, sargassum fusiforme polysaccharide is extracted by utilizing an ultrasonic-assisted hot water method, and process parameters such as hot water extraction time, ultrasonic time, ultrasonic power and a materiaLliquid ratio are optimized by adopting a response surface method, so that the yield of crude polysaccharide reaches 26%.

Description

technical field [0001] The invention belongs to the field of sugar extraction, in particular to a hijiki polysaccharide and an extraction method thereof. Background technique [0002] Hijiki belongs to the Sargassum family of Fucus, Phaeaceae, and is distributed in the shallow waters of Liaodong Peninsula, Fujian, and Guangdong in my country, and it also grows in Japan and South Korea. Hijiki is rich in polysaccharides, food cellulose, multivitamins, minerals and trace elements, and has 18 important amino acids needed by the human body. Hijiki polysaccharide is an acidic polysaccharide with various pharmacological activities, mainly composed of alginic acid and fucoidan, and its content in dry hijiki is 16%-24%. As one of the main effective activities, Sargassum polysaccharide (SFPS) is an effective and non-toxic natural compound, and it is a better choice as a nano-selenium modifier. [0003] At present, the extraction methods of hijiki polysaccharides are mainly hot wate...

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Problems solved by technology

For example: the yield of crude polysaccharides extracted by traditional hot water extraction by Chen Shaoyuan was only 8.6%; the yield of polysaccharides by microwave extraction by Tang Zhihong was 15.6%; the yield of polysaccharides extracted by Wang Qin and others reached 39.3%, but the activity of polysaccharides was destroyed; China Patent CN109369819A discloses an extraction method for enzymatically extracting hijiki polysaccharides, the polysaccharide extraction rate reaches more than 40%, but there are disadvantages such as high extraction cost

Existing technologies for extracting hijiki polysaccharides generally have shortcomings such as long time-consuming, high energy consumption, and high equipment requirements, so they are still in the stage of small-scale extraction in the laboratory, and have not been used for mass production in factories

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