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Construction method of high-fat diet induced anterior ischemic optic neuropathy pigment rabbit model and application

An optic neuropathy and ischemia technology, applied in the application, additional food elements, pharmaceutical formulations, etc., can solve the problem of difficult disease model establishment, cannot truly reflect the occurrence process and natural course of human NAION, and the cost of primates is high. Ethics and other issues to achieve the effect that is conducive to industrial application

Pending Publication Date: 2021-01-15
WEST CHINA HOSPITAL SICHUAN UNIV +1
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, some studies believe that this photochemically induced animal model cannot truly reflect the occurrence and natural course of human NAION, and this method mainly destroys the central retinal artery that supplies the inner retina, rather than the deep ciliary artery, during the modeling process. posterior artery
In addition, due to the high cost of primates and animal ethics issues, it is difficult to be widely used in the establishment of disease models

Method used

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  • Construction method of high-fat diet induced anterior ischemic optic neuropathy pigment rabbit model and application
  • Construction method of high-fat diet induced anterior ischemic optic neuropathy pigment rabbit model and application
  • Construction method of high-fat diet induced anterior ischemic optic neuropathy pigment rabbit model and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Construction and verification of NAION pigmented rabbit model

[0033] 1. Method

[0034] 1.1 Modeling

[0035] Divide 16-week-old adult male Qingzilan rabbits with a body weight of 3.5±0.5kg into two groups, each with 10 rabbits. Feeding a high-fat diet (the rabbit maintains the feed with 10% lard by weight and 2% cholesterol by weight), the amount that each rabbit is fed every day is 40-60g (10-20g / kg body weight) in the morning and 60 in the afternoon. -90g (15-30g / kg body weight).

[0036] The maintenance feed is relative to the breeding feed, the protein and energy level of the former is lower than that of the breeding feed, so as to prevent the animal from being overweight in the maintenance period (from the beginning of weaning separation to the redevelopment of sexual organs). Nutrient composition of maintenance feed: 23.5% (w / w) protein, 11% (w / w) fat and 65.5% (w / w) carbohydrate; energy: 2.7kcal / g.

[0037] 1.2 Physiological index examination

...

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Abstract

The invention discloses a construction method of an anterior ischemic optic neuropathy pigment rabbit model, and belongs to the field of ophthalmic disease animal models. The method comprises the following steps of adding 10%+ / -3% by weight of lard and 1%+ / -0.5% by weight of cholesterol into rabbit maintenance feed, and continuously feeding pigment rabbits for more than 24 weeks. The method provided by the invention can enable the animals to generate symptoms of non-arterial inflammatory anterior ischemic optic neuropathy only by diet control, is simple and convenient, and is easy for industrial application.

Description

technical field [0001] The invention belongs to the field of ophthalmic disease animal models. Background technique [0002] Anterior ischemic optic neuropathy (AION) is the most common acute blinding optic neuropathy in people over 50 years old, and it has always been the focus and difficulty of ophthalmology clinical work. AION is caused by insufficient blood supply to the anterior optic nerve (i.e. the optic disc) due to circulatory disturbance of the posterior ciliary artery, often involving both eyes, with varying intervals of onset; clinically, it can be divided into non-arteritic anterior ischemic optic neuropathy (NAION) and arteritic anterior ischemic optic neuropathy (AAION). The former accounts for about 85% of all AION cases, while the latter is less common and usually affects people over the age of 70. At present, the pathogenesis of NAION pathophysiology is unclear and controversial, and the generally recognized risk factor is "optic disc crowding". Histologi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A23K50/50A23K20/158A23K20/168A61K49/00
CPCA01K67/02A23K50/50A23K20/158A23K20/168A61K49/0008
Inventor 王钰娇岑小波陈大年
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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