Application of Paracoccus sediminis CGMCC 1.12681 in preparation of biopesticide
A CGMCC1.12681, biopesticide technology, applied in the application field of preparation of biopesticides, can solve the problems of no immediate effect of pests and diseases, slow drug effect, high temperature and humidity requirements, etc.
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Embodiment 1
[0039] A kind of preparation method of biopesticide, concrete steps are as follows:
[0040] (1) First inoculate and cultivate the paracoccus paracoccus CGMCC1.12681 to obtain a fermentation broth, and then process it to make bacterial powder;
[0041] (2) Then, the discarded Hericium erinaceus rod is crushed into 100-mesh powder, then the powder, the bacterial powder obtained in step (1), acrylamide and sodium acrylate are added to water, and the ultrasonic vibration is uniform to obtain the premix;
[0042] (3) Then add a crosslinking agent and a photoinitiator to the premixed liquid and mix evenly, react with ultraviolet radiation, filter, wash, and dry to obtain a xerogel;
[0043] (4) Finally, add the dry gel into 1-octyl-3-methylimidazolium tetrafluoroborate, let it stand for 3 hours, and filter to obtain the biopesticide.
[0044] The specific method of step (1) is: first inoculate the paracoccus paracoccus CGMCC1.12681 in the PDA medium, the inoculum size is 3% (volum...
Embodiment 2
[0054] A kind of preparation method of biopesticide, concrete steps are as follows:
[0055] (1) First inoculate and cultivate the paracoccus paracoccus CGMCC1.12681 to obtain a fermentation broth, and then process it to make bacterial powder;
[0056] (2) Then the discarded Hericium erinaceus rods were crushed into 200-mesh powder, and then the powder, the bacterial powder obtained in step (1), acrylamide and sodium acrylate were added to water, and ultrasonically oscillated evenly to obtain a premix;
[0057] (3) Then add a crosslinking agent and a photoinitiator to the premixed liquid and mix evenly, react with ultraviolet radiation, filter, wash, and dry to obtain a xerogel;
[0058] (4) Finally, add the dry gel into 1-octyl-3-methylimidazolium tetrafluoroborate, let it stand for 2 hours, and filter to obtain the biopesticide.
[0059] The specific method of step (1) is: first inoculate the paracoccus paracoccus CGMCC1.12681 in the PDA medium, the inoculum size is 4% (vol...
Embodiment 3
[0069] A kind of preparation method of biopesticide, concrete steps are as follows:
[0070] (1) First inoculate and cultivate the paracoccus paracoccus CGMCC1.12681 to obtain a fermentation broth, and then process it to make bacterial powder;
[0071] (2) Then the discarded Hericium erinaceus rods were crushed into 200-mesh powder, and then the powder, the bacterial powder obtained in step (1), acrylamide and sodium acrylate were added to water, and ultrasonically oscillated evenly to obtain a premix;
[0072] (3) Then add a crosslinking agent and a photoinitiator to the premixed liquid and mix evenly, react with ultraviolet radiation, filter, wash, and dry to obtain a xerogel;
[0073] (4) Finally, add the dry gel into 1-octyl-3-methylimidazolium tetrafluoroborate, let it stand for 2 hours, and filter to obtain the biopesticide.
[0074] The specific method of step (1) is: first inoculate the paracoccus paracoccus CGMCC1.12681 in the PDA medium, the inoculum size is 3.5% (v...
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