Unmarked multi-modal microscopic system suitable for researching organelle interaction network

Abstract

The invention discloses an unmarked multi-modal microscopic system suitable for researching an organelle interaction network. The unmarked multi-modal microscopic system comprises a Raman channel, a phase channel, a fluorescence channel, a precision displacement table, an illumination light source and a plurality of lasers; the Raman channel comprises a Raman spectrometer, a 2D galvanometer and a1D galvanometer, and is used for providing a rapid scanning Raman spectrum signal; and the phase channel is used for recording morphological information of the unmarked organelles and recording phaseimages. According to the invention, a high space-time resolution phase technology and a rapid Raman scanning technology are fused, so that rapid detection of forms of various organelles is realized, and meanwhile, real-time tracking of the moving organelles is realized, and Raman spectrum information of the moving organelles is collected, so that material exchange between a cell structure and other cell structures is analyzed. Compared with the existing fluorescence technology, the system has the advantages that the forms of various organelles can be observed at the same time, the exchange ofvarious substances can be detected in situ, and the dynamic change characteristics of the network interaction of the organelles can be described under the condition of minimum interference.

Description

technical field [0001] The invention relates to biological microscopic imaging technology, in particular to a label-free multi-modal microscopic system suitable for the study of cell organelle interaction network. Background technique [0002] Cells are the basic unit of life activities, and organelles are the functional departments of cells, performing life activities closely related to cell fate, such as metabolism, stress, autophagy and so on. Cells contain more than ten kinds of organelles, and each organelle forms an organelle interaction network through mutual cooperation and close contact to realize material exchange, and at the same time transmit signal factors to participate in regulation. The disorder of organelle network function will cause various metabolic diseases (obesity, diabetes, senile dementia, etc.), and even the occurrence and development of tumors. At present, the research on the form, function and mechanism of organelle interaction is still in its in...

AI Technical Summary

Problems solved by technology

[0003] At present, the observation of the dynamic process of the interaction network mainly relies on fluorescence microscopy, but there are problems in fluorescence microscopy, such as phototoxicity, short observation time, difficulty in multi-object labeling, and prior knowledge required for fluorescent labeling.

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