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Cholesterol silicon phthalocyanine (Chol-SiPc) nano-systems capable of serving as living-cell cholesterol zone and Golgi body probe, preparation method for Chol-SiPc nano-systems and application of Chol-SiPc nano-systems

A Golgi and cholesterol technology, applied in the fields of nanotechnology, chemical instruments and methods, and nanotechnology for materials and surface science, can solve the problems of high price, easy bleaching, incomplete clarity of Filipin staining cholesterol, etc. Aggregation behavior, improvement of stability, and effect of improving the efficiency of reactive oxygen species generation

Pending Publication Date: 2020-01-17
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is not entirely clear whether Filipin staining accurately reflects the distribution of cholesterol, especially in intracellular sites that are not easily accessible and / or susceptible to fixation techniques
The Golgi apparatus is the main organelle in the cholesterol region, and its localization mainly includes the commercial dye Glogi-Tracker Red, but it is expensive, and has poor selectivity in organelle imaging and is easy to bleach

Method used

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  • Cholesterol silicon phthalocyanine (Chol-SiPc) nano-systems capable of serving as living-cell cholesterol zone and Golgi body probe, preparation method for Chol-SiPc nano-systems and application of Chol-SiPc nano-systems
  • Cholesterol silicon phthalocyanine (Chol-SiPc) nano-systems capable of serving as living-cell cholesterol zone and Golgi body probe, preparation method for Chol-SiPc nano-systems and application of Chol-SiPc nano-systems
  • Cholesterol silicon phthalocyanine (Chol-SiPc) nano-systems capable of serving as living-cell cholesterol zone and Golgi body probe, preparation method for Chol-SiPc nano-systems and application of Chol-SiPc nano-systems

Examples

Experimental program
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Effect test

specific Embodiment 1

[0024] 1) Dichlorosilicon (IV) phthalocyanine (SiPcCl 2 )Synthesis

[0025] Add 1,3-diiminoisoindoline (7.28 g, 50.15 mmol), silicon tetrachloride (8.3 mL) and quinoline (83 mL) respectively into a three-necked flask, stir and reflux for 30 min at 220 °C , cooled to room temperature, poured into 500 mL of methanol solution, stirred and allowed to stand for about 1 hour, filtered, and the filter residue was washed with 35 mL each of acetone, methanol, dichloromethane, methanol and other solvents, and dried to obtain a purple-red solid (2 Chlorosilicon (IV) phthalocyanine) 3.6759 g, yield 48.62%.

[0026] ) Synthesis of cholesteryl silicon phthalocyanine (Chol-SiPc)

[0027] Cholesterol (CAS: 57-88-5) (0.115 g, 0.3 mmol), dichlorosilicon(IV) phthalocyanine (0.0611 g, 0.1 mmol) from step 1) Anhydrous potassium carbonate (0.276 g, 2 mmol) Add 20 mL of toluene to a 100 mL reaction flask, and reflux for 24 hours at 120 °C. Cooled to room temperature, filtered, and the filtrate w...

specific Embodiment 2

[0034] Concrete steps are the same as embodiment one: K in process 2) 2 CO 3 (0.09 g, 0.654 mmol) was changed to NaH (0.01 g, 0.654 mmol), and other reaction conditions were the same. 6.6 mg of blue-purple solid (cholesteryl silicon phthalocyanine (Chol-SiPc)) was obtained with a yield of 2.4%.

[0035] Process 2) K 2 CO 3 (0.09 g, 0.654 mmol) was changed to pyridine (0.05 g, 0.654 mmol), and other reaction conditions were the same. 5.8 mg of blue-purple solid (cholesteryl silicon phthalocyanine (Chol-SiPc)) was obtained with a yield of 1.46%.

[0036] ) Real-time tracking of breast cancer cells MCF-7 and MDA-MB-231 on FITC-DSPE@Chol-SiPc

[0037] MCF-7 and MDA-MB-231 cells in the logarithmic growth phase were taken, digested, collected by centrifugation, resuspended and counted, and approximately 1×10 4 The concentration was placed in a 3.5 cm confocal dish, placed at 37°C, 5% CO 2 Cultivate overnight (20-24 h) in a constant temperature incubator. The next day, the cu...

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Abstract

The invention discloses cholesterol silicon phthalocyanine (Chol-SiPc) nano-systems capable of serving as a living-cell cholesterol zone and a Golgi body probe, a preparation method for the Chol-SiPcnano-systems and an application of the Chol-SiPc nano-systems. The Chol-SiPc nano-systems are amphiphilic block copolymer loaded Chol-SiPc nano-systems and are self-assembled from amphiphilic block copolymers DSPE-mPEG2000(1,2-distearyl-sn-glyceryl-3-ethanol amine phosphate-N-[methoxy(polyethylene glycol)-2000]) and Chol-SiPc through a cosolvent method. The invention discloses cholesteryl axial SiPc complexes, a preparation method therefor and an application of the cholesteryl axial SiPc complexes. The cholesteryl axial SiPc complexes are Chol-SiPc complexes prepared from cholesterol and dichloro silicon (IV) phthalocyanine. The invention further discloses tracing of the Chol-SiPc nano-systems in mammary cancer cells and positioning of the Chol-SiPc nano-systems in cholesterol zones and Golgi bodies. Particularly, the Chol-SiPc nano-systems serve as photosensitizers for photodynamic therapy.

Description

technical field [0001] The invention belongs to the field of molecular probes, in particular to a cholesteryl silicon phthalocyanine nanosystem that can be used as a cholesterol region and a Golgi body probe and its preparation method and application. The amphiphilic block copolymer supports a cholesterol silicon phthalocyanine nanosystem as a living cell Cholesterol domain and Golgi probes and photosensitizers for photodynamic therapy. Background technique [0002] Photodynamic therapy (PDT) is considered to be a promising approach for the treatment of cancer and non-cancer diseases with clinical application. It uses the combined action of photosensitizers (PSs), light, and oxygen to generate reactive oxygen species (ROS) to destroy cancer cells. PDT is spatiotemporally selective for cancer therapy because the ROS generated by PSs only occurs at the site exposed to light, while the resulting chemical reactions occur within tens of nanometers around this area. However, the...

Claims

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Application Information

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IPC IPC(8): C09K11/02C07J51/00C09K11/06B82Y30/00G01N21/64A61K41/00A61P35/00
CPCC09K11/025C07J51/00C09K11/06B82Y30/00G01N21/6402G01N21/6428G01N21/6486A61K41/0071A61P35/00C09K2211/1011C09K2211/1074G01N2021/6432
Inventor 彭亦如陈秀琴黄义德郭秋梅林芃臻
Owner FUJIAN NORMAL UNIV
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