Preparation method of a mesoporous manganese dioxide nanometer drug-loading system with photodynamic therapy switching effect and molecular recognition

Abstract

The invention relates to a preparation method for a mesoporous manganese dioxide nanometer drug delivery system with photodynamic therapy switch effect and molecular recognition effect. The mesoporousmanganese dioxide nanometer drug delivery system can effectively overcome medication problems in photodynamic therapy of tumors. The preparation method comprises the following steps: dissolving sodium dodecyl benzene sulfonate and KMnO4 in ultrapure water, adding a nitric acid solution, carrying out centrifugation, and drying and calcining a precipitate so as to obtain mesoporous MnO2 nanoparticles; weighing a methanol solution of hematoporphyrin monomethyl ether, adding methanol solution of the mesoporous MnO2 nanoparticles, and successively carrying out uniform mixing, ultrasonic treatmentand spin-drying so as to obtain mesoporous MnO2 nanoparticles loaded with the photosensitizer hematoporphyrin monomethyl ether; weighing ssDNA 5'-HS-TTTCCCAGTTGATCCTTTGGATACCCTGGG and preparing hairpin nucleic acid aptamers; and adding the aptamers into the mesoporous MnO2 nanoparticles loaded with the photosensitizer hematoporphyrin monomethyl ether for a reaction to allow as many aptamers as possible to be immobilized onto the surfaces of the mesoporous MnO2 nanoparticles loaded with the photosensitizer hematoporphyrin monomethyl ether. The method of the invention is simple in process, easyto operate, rich in raw materials, low in cost, good in use effect, and capable of preventing the photosensitivity of PDT.

Description

technical field [0001] The invention relates to medicine, in particular to a preparation method of a mesoporous manganese dioxide nano drug-carrying system with photodynamic therapy switch effect and molecular recognition function. Background technique [0002] Tumor is a disease that seriously threatens human health and is difficult to cure. Currently, the main treatments for tumors include surgery, chemotherapy and radiotherapy. Since the 1990s, tumor photodynamic therapy has become a research hotspot, mainly focusing on the tumor-specific transport of photosensitizers, the mechanism of photodynamic therapy on tumors, and its synergistic effect with other treatments. [0003] The mechanism of tumor photodynamic therapy is also an important direction of tumor photodynamic therapy. Due to the very limited lifespan and movement distance of reactive oxygen species (ROS) generated in photodynamic therapy, only 20nS, 200nm, most ROS do not cause damage to the effective parts of...

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Problems solved by technology

[0005] However, there are still some problems in photodynamic therapy at present. Most photosensitizers such as HMME are water insoluble drugs, which are difficult to be directly applied in the body; Efficient distribution of tumor cells, subcellular organelles); tumor cells have a certain oxidative stress regulation effect, reducing the killing effect of active oxygen;

[0006] More importantly, in previous studies, it was found that commonly used photosensitizers such as HMME and CE6 are difficult to be metabolized and cleared in the body, and accumulate in large quantities in the body, resulting in severe photosensitivity toxicity. Toxicity, the patient needs to be completely protected from light for about 4 weeks, which undoubtedly brings great pain to the patient

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