Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of Ulexin C detection reagent in preparation of COPD diagnosis kit

A kit and reagent technology, applied in the field of lung disease detection, can solve problems such as UlexinC that have not yet been seen, and achieve the effect of rapid diagnosis

Active Publication Date: 2021-01-29
WEST CHINA HOSPITAL SICHUAN UNIV
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, there is no report on the relationship between Ulexin C and COPD

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of Ulexin C detection reagent in preparation of COPD diagnosis kit
  • Application of Ulexin C detection reagent in preparation of COPD diagnosis kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Detection of Ulexin C in serum of COPD patients

[0026] 1. Subjects under inspection

[0027] There were 60 COPD patients (Group A) and 30 healthy controls (Group B). All subjects were informed and agreed before the experiment.

[0028] 2. Method

[0029] The serum of the subjects was subjected to sample pretreatment, metabolite extraction, LC-MS full-scan detection, data preprocessing and statistical analysis. Based on the metabolomics function of the UPLC-VION IMS Q-Tof high-resolution mass spectrometer, combined with the metabolomics data processing software Progenesis QI v2.3, the qualitative and relative quantitative analysis of the raw data is performed, and the raw data is standardized and preprocessed.

[0030] Specific experimental steps:

[0031] (1) Sample pretreatment

[0032] 1) Take out the sample stored at -80°C, thaw at room temperature, pipette 100 μL of serum, add internal standard (L-2-chlorophenylalanine, 0.3mg / mL; C-17, 0.01mg / mL, (...

Embodiment 2

[0052] Embodiment 2 Kit of the present invention

[0053] 1. The composition of kit of the present invention

[0054] Methanol, formic acid, acetonitrile and L-2-chlorophenylalanine.

[0055] 2. How to use the kit

[0056] Experimental operation is the same as Section 2 of Example 1.

[0057] When the detected value of Ulexin C in serum is above 0.064, it can be identified as COPD; otherwise, it can be identified as non-COPD.

[0058] To sum up, the kit of the present invention can realize the auxiliary diagnosis of COPD through the quantitative detection of Ulexin C, can provide clinicians with valuable reference information simply and quickly, is convenient for prescribing the right medicine, and has a good application prospect.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an application of a Ulexin C detection reagent in preparation of a COPD diagnostic kit, and belongs to the field of lung disease detection. According to the invention, rapid auxiliary diagnosis of COPD can be realized by detecting the content of Ulexin C in serum.

Description

technical field [0001] The invention belongs to the field of lung disease detection. Background technique [0002] Chronic obstructive pulmonary disease (COPD) is a chronic bronchitis and / or emphysema characterized by airflow obstruction, which can further develop into pulmonary heart disease and respiratory failure. The morbidity rate and mortality rate of COPD are very high, and the global incidence rate of over 40 years old has been as high as 9% to 10%. [0003] The exact etiology of COPD is unclear, and it is generally believed that factors related to the occurrence of chronic bronchitis and obstructive emphysema may be involved in the pathogenesis of COPD. The risk factors that have been discovered can be roughly divided into two categories: external factors (ie, environmental factors) and internal factors (ie, individual susceptibility factors). External causes include smoking, inhalation of dust and chemicals, air pollution, respiratory infections, and lower socioe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 申永春秦江月王浩陈磊文富强
Owner WEST CHINA HOSPITAL SICHUAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com