Determination method of a traditional Chinese medicine composition and determination method of efficacy thereof
A technology for determining methods and compositions, which can be used in alternative medicines, genomics, proteomics, etc., and can solve the problems of difficult quantitative evaluation of therapeutic effects.
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Embodiment 1
[0095]Example 1 Chinese medicine combination for the treatment of novel coronavirus infection
[0096]1. Obtain disease status data of new coronavirus infection
[0097]Retrieve publicly published data on new coronavirus infections on the NCBI official website GEO database. GSE147507 contains the transcription data of the new coronavirus infected Calu-3 cell line. The detection method is RNA-seq and the platform used is IlluminaNextseq-500. In the KEGG pathway database, in the "Infectious disease: viral" section, there are a total of 11 pathways related to viral infections, such as influenza A, hepatitis B, hepatitis C, and HIV infection.
[0098]The GSE147507 data was analyzed for the fold change of gene expression, and the DEseq2 method was used to obtain the fold change of gene expression. Gene set enrichment analysis (GSEA) was used to carry out the enrichment analysis of signal pathways, and the disorders of the disease state in 11 pathways were obtained. Where FDR<0.25 is considered to...
Embodiment 2
[0122]Example 2 Evaluation of the curative effect of prescriptions for the treatment of new coronavirus infection
[0123]1. In response to the new coronavirus infection, the state has issued the "New Coronavirus Infection Pneumonia Diagnosis and Treatment Plan (Trial Version 6)", which includes 22 prescriptions or patent medicines, which are:
[0124]Table 5 Prescriptions or patent medicines involved in the "Diagnosis and Treatment Plan for Pneumonia of Novel Coronavirus Infection (Trial Version 6)"
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[0127]①Classification standards for light, ordinary, heavy, and critical types:
[0128]Mild: The clinical symptoms are mild, and there is no pneumonia in imaging;
[0129]Common type: symptoms such as fever and respiratory tract, and imaging manifestations of pneumonia.
[0130]Heavy: Meet any of the following:
[0131]1. Shortness of breath occurs, RR≥30 times / min
[0132]2. In the resting state, the oxygen saturation is ≤93%
[0133]3. Partial pressure of arterial blood pressure (PaO2) / inhaled ox...
Embodiment 3
[0154]Example 3 Chinese medicine prescription for treating liver insufficiency
[0155]1. Obtain disease status data of liver insufficiency
[0156]Retrieve publicly published data on liver insufficiency on the NCBI official website GEO database. GSE141864 is a study on acute septic shock and multiple organ failure caused by Neisserial meningitis infection, including the complete transcription of liver tissue after death. Group data, detection method is microarray chip, and the platform used is Affymetrix GeneChipHuman Transcriptome 2.0arrays.
[0157]The GSE141806 data was analyzed for the fold change in gene expression, and the DEseq2 method was used to obtain the fold change in gene expression. Gene set enrichment analysis (GSEA) was used to carry out the enrichment analysis of the signal pathway, and the disorder in 43 liver function-related pathways was obtained. The standardized enrichment score can characterize the disorder direction of the pathway, and NES is positive It means that t...
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