Integrated PCR instrument and control method thereof

Abstract

The invention relates to a PCR instrument and a control method thereof. The PCR instrument structurally comprises a base assembly, a bearing platform assembly, a bearing platform driving mechanism, apushing platform assembly, a heating module, a fluorescence detection module, a liquid storage container and an air source; the heating module is arranged on the base assembly; the bearing platform assembly is transversely arranged on the base assembly in a sliding mode; a chip bearing platform moving up and down is further arranged on the bearing platform assembly and located above the heating module; the pushing platform assembly is arranged above the bearing platform assembly and opposite to the heating module; a plurality of nozzles are arranged at the bottom of the pushing platform assembly; the nozzles are connected with the liquid storage container and the air source separately; the fluorescence detection module is arranged below the heating module; the heating module is provided with a light hole corresponding to the fluorescence detection module; the light hole penetrates through the upper side and the lower side of the heating module; the heating module is provided with at least two heating areas; and a heat insulation belt is arranged between every two heating areas. According to the instrument, liquid injection, double-temperature heating and optical detection are combined together, so that the polymerase chain reaction of a detected sample can be completed by single equipment.

Description

Technical field[0001]The invention relates to a PCR instrument, in particular to an integrated PCR instrument and a control method thereof.Background technique[0002]Polymerase chain reaction (Polymerase Chain Reaction, PCR) is a molecular biology technology used to amplify specific DNA fragments. It can be regarded as a special DNA replication in vitro. The biggest feature of PCR is that it can The DNA has increased dramatically.[0003]PCR uses DNA to become single-stranded when it is denatured at a high temperature of 95°C in vitro. At low temperature (usually around 60°C), the primer and single-strand are combined according to the principle of base complementary pairing, and then the temperature is adjusted to the DNA polymerase optimum At the reaction temperature (about 72°C), the DNA polymerase synthesizes the complementary chain along the direction of phosphate to five-carbon sugar.[0004]The basic principle of current digital PCR analysis is to distribute the sample into a large...

AI Technical Summary

Problems solved by technology

However, the current technology does not have an effective positioning scheme, so that the microfluidic chip is prone to movement during the liquid injection process, resulting in inaccurate positioning

Reduce the efficiency of PCR, and easily lead to leakage or contamination of PCR samples

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