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Application of SNP locus of CDA gene

A site and gene technology, applied in the field of CDA gene SNP site, can solve the problem of rare antihypertensive drugs and achieve a highly specific effect

Active Publication Date: 2021-02-05
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, pharmacogenomics research on antihypertensive drugs is still rare. Further research on molecular markers to predict the efficacy of antihypertensive drugs is of great significance for the treatment and diagnosis of hypertension.

Method used

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  • Application of SNP locus of CDA gene
  • Application of SNP locus of CDA gene
  • Application of SNP locus of CDA gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1. Using the Illumina iSelect HD high-throughput chip to conduct a randomized, double-blind, controlled, multi-center follow-up period of up to 4 years in a large-scale clinical trial "Felodipine Treatment of Hypertension Study" samples were carried out on 3004 genes in 406 genes For the detection of genetic variation, a total of 1,197 samples and 76,392 blood pressure phenotype data were included. The specific experimental process is as follows: figure 1 shown.

[0042] CDA(rs818202)( figure 2), IDE (rs35426658), CBR3-AS1 (rs2835286), CYP4F2 (rs2108622), CBR1 (rs2835265), FMO3 (rs1736557), CYP20A1 (rs1048013), CES2 (rs4783745), FMO6P (rs1736565) The correlation between therapeutic effects was corrected by multiple testing (P-5 ),has statistical significane.

[0043] Subsequently, in the verification sample, the genetic polymorphism of CDA rs818202 was a site that could still significantly affect the efficacy of antihypertensive drugs ( image 3 ). Therefore, we on...

Embodiment 2

[0049] CDA gene variation detection method: PCR-direct sequencing method (PCR-sequencing) was used for genotyping.

[0050] 1. PCR primer sequence

[0051] Using Primer Premier 5.0 primer design software, according to the genome sequence of 500 bp before and after the CDA gene polymorphism site (rs818202) in the NCBI database (1001 bp in total) as the reference sequence, the length of the PCR amplification product was limited to 100-500 bp, and the software designed The primers were verified in the NCBI database for the specificity of the amplified products.

[0052] Table 1 CDA gene PCR and sequencing primers

[0053]

[0054] 2. PCR amplification reaction system

[0055] Table 2 PCR amplification reaction system

[0056]

[0057] 3. PCR optimization conditions

[0058] Step 1: Pre-denaturation at 95°C for 5 minutes

[0059] The second step: a total of 36 loops

[0060] Denaturation at 95°C for 30 seconds

[0061] Annealing at 61°C for 45 seconds

[0062] 72°C e...

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Abstract

The invention relates to the technical field of biology, in particular to application of an SNP locus of a CDA gene. Researches show that the SNP locus rs818202 of CDA can significantly affect the long-term antihypertensive effect of felodipine, and a genetic model constructed on the basis of rs818202 can better predict the antihypertensive effect. A kit prepared by taking the SNP locus of the CDAas a marker can achieve a good prediction effect on the medication effect of an antihypertensive drug.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of the CDA gene SNP site. Background technique [0002] Hypertension is a clinical syndrome characterized by elevated systemic arterial pressure, or accompanied by functional or organic damage to heart, brain, kidney and other organs. major risk factors. Patients often show symptoms such as dizziness, headache, vertigo, tinnitus, insomnia, and fatigue. [0003] The treatment of hypertension mainly includes improving lifestyle and drug therapy, and most patients may need to take drugs for life. At present, there are many kinds of antihypertensive drugs available for clinical selection, but studies have shown that the antihypertensive efficacy of various antihypertensive drugs varies greatly among individuals. As an important part of individualized medicine or precision medicine, pharmacogenetics and pharmacogenomics have shown that genetic factors play an important ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/106C12Q2600/156
Inventor 罗建权任欢张伟
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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