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Corn ear length gene, molecular markers and applications

A technology of molecular markers and ears of corn, applied in the field of molecular genetics, can solve problems such as being easily affected by environmental conditions and complex genetic mechanisms, and achieve the effect of increasing corn yield and high transcriptional activity

Active Publication Date: 2021-02-19
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these traits are quantitative traits controlled by polygenes, the genetic mechanism is relatively complex, and they are easily affected by environmental conditions.

Method used

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  • Corn ear length gene, molecular markers and applications
  • Corn ear length gene, molecular markers and applications
  • Corn ear length gene, molecular markers and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Example 1 Mapping of Ear Length QTL in Maize

[0073] The invention locates a corn ear length QTL from a corn related population. The maize related population collected 527 maize inbred lines with extensive phenotypic variation and genetic variation from 54 from the United States, 235 from the International Maize and Wheat Improvement Center, and 238 from China (for the construction method of the related population, refer to: Yang X, Gao S, Xu S, Zhang Z, Prasanna BM, Li L, Li J, Yan J. 2010. Characterization of a global germplasm collection and its potential utilization for analysis of complex quantitative traits in maize. Molecular Breeding 28(4):511 -526.). In 2009, Xi'an, Sanya, and Yunnan in China, and in Guangxi, and Yunnan in 2010, the phenotypic value of maize ear length in the associated population was investigated, and the best linear unbiased predictions (BLUP) was used to evaluate the genetic effect, and the BLUP was obtained. Corn ear length phenotype val...

Embodiment 2

[0075] Cloning and functional verification of the corn ear length gene of embodiment 2

[0076] Further analysis of the genomic information of the qEL1 interval found that within this QTL interval, there are 4 genes with functional annotations (based on the v2 version of the maize B73 reference genome), which are I: GRMZM2G329040, II: GRMZM2G703565, III: GRMZM2G008490 and IV: AC208571 .4-FG001. Take corn ear samples of short-ear and long-ear corn inbred lines YU87-1 and BK respectively (the sampling period is when the young ear grows to about 2-5mm), extract RNA and reverse transcribe it into cDNA (operation method Refer to the instructions of the Huayueyang kit), and then use real-time fluorescent quantitative PCR to detect the expression levels of the above four candidate genes in YU87-1 and BK young ear tissues, and use the expression level of the corn internal reference gene Zm00001d010159 as a reference (for primers, see Table 1). For the detection of each gene expressi...

Embodiment 3

[0091] Example 3 Analysis of ZmEL1 Gene Transcript and Coding Protein Sequence

[0092] According to the annotation information on the maize reference genome, the amino acid sequence of the protein encoded by ZmEL1 (GRMZM2G00849) is shown in SEQ ID NO.1, and the genome sequence is shown in SEQ ID NO.2. The gene function is annotated as an unknown protein, and there is no public information showing that the gene controls the corn ear length traits.

[0093] According to the genome information of the maize v4 version in the Gramene database, it is found that there are a total of 20 predicted transcripts of the ZmEL1 gene, and it is not known which one is the true transcript of the gene. Combined with the information of the maize V3 version genome, the present invention designs 3 pairs of primers from ATG to TGA for each of the 10 transcripts in the v4 version, and uses Yu87-1, BK and B73 corn 2-5mm young ears Using the cDNA as a template for PCR amplification, it was found that...

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Abstract

The invention relates to a corn ear length gene ZmEL1, a promoter, molecular markers linked to the promoter and applications of the gene and the molecular markers in screening ear length traits and cultivating long ear varieties, and belongs to the field of molecular genetics. The disclosed corn ear length gene ZmEL1 is characterized in that the nucleotide sequence of the gene is shown as any of SEQ ID NO. 2- SEQ ID NO.3; and an encoded amino acid sequence is shown as SEQ ID NO.1. Meanwhile, a promoter sequence (SEQ ID NO.4- SEQ ID NO.7), the molecular markers S2, S3 and S4 closely linked to the promoter, detection primer pairs of the markers (SEQ ID NO.8- SEQ ID NO.16) and a detection method are also disclosed. Further, a method for screening the corn ear length traits and increasing thelength of corn ears by utilizing the molecular markers is provided.

Description

technical field [0001] The invention relates to a corn ear length gene ZmEL1, a promoter and a molecular marker linked thereto and its application in screening corn ear length traits and cultivating long-ear corn varieties, belonging to the field of molecular genetics. Background technique [0002] Corn is an important food and feed crop in our country. In addition, it is also an indispensable raw material for many industries such as medical treatment, chemical industry and brewing, and plays an important role in our national economy. The yield of maize is the result of the coordinated development of factors such as the number of ears per unit area and the yield per ear. Therefore, genetic research on the plant type and ear traits that determine these factors is of great significance to the genetic improvement of yield. However, most of these traits are quantitative traits controlled by polygenes, the genetic mechanism is relatively complex, and they are easily affected by e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/113C12Q1/6895C12N15/11A01H1/04C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12Q1/6895A01H1/04C12N15/8261C12Q2600/13C12Q2600/156
Inventor 严建兵罗芸张明亮刘喻李文强
Owner HUAZHONG AGRI UNIV
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