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Application of miR-995 in prevention and treatment of lepidoptera pests

A mir-995, 1.mir-995 technology, applied in the application field of miR-995 in the control of lepidopteran pests, can solve the problem that miR-995 regulates the development of insect wings, which has not been reported, and achieves significant economic benefits and social benefits, the effect of reducing wing area

Active Publication Date: 2021-02-26
SHANDONG AGRICULTURAL UNIVERSITY
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] miR-995 is a member of many miRNAs. Currently, there are few studies on the function of miR-995, and there is no report on the regulation of miR-995 on insect wing development.

Method used

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  • Application of miR-995 in prevention and treatment of lepidoptera pests
  • Application of miR-995 in prevention and treatment of lepidoptera pests

Examples

Experimental program
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Effect test

Embodiment 1

[0034] 1. Overexpression of miR-995 significantly reduces the wing area of ​​Drosophila

[0035] UAS-GFP and UAS-miR-995 male flies were crossed with En-gal4 virgin flies specifically expressed in the posterior (P) region of Drosophila wings, and the adult wing phenotypes of the offspring were observed. The wings were photographed with a stereo microscope, and the areas of the P area and anterior (A) area were measured by ImageJ software, and the P / A ratio was calculated, compared with the control group, and statistically analyzed. Due to the difference in the individual size of offspring male Drosophila and female Drosophila, this study carried out statistics on the female and male individuals separately. The results of this study only show the wings and statistical results of male Drosophila adults.

[0036] The result is as figure 1 As shown, the P / A value of the control group was 1.3, and the P / A value decreased to 0.7 after miR-995 was overexpressed. Further statistical...

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Abstract

The invention discloses an application of miR-995 in prevention and treatment of lepidoptera pests. Research finds that overexpression of miR-995 can significantly reduce the fin area and inhibit findevelopment; further research finds that the action mechanism of the miR-995 is as follows: the miR-995 directly inhibits the expression of a target gene diap1 of a Hippo signaling pathway, and further inhibits the development of wings. The miR-995 can significantly inhibit insect wing development, and important target gene resources are provided for RNAi-mediated pest biological control by artificially synthesizing the miR-995 or analogues thereof or utilizing transgenic plants expressing the miR-995. According to the invention, theoretical and practical significance is provided for prevention and treatment of winged insects, and meanwhile, the method has remarkable economic benefits and social benefits.

Description

technical field [0001] The invention relates to the fields of agricultural biotechnology and pest control, in particular to the application of miR-995 in the control of lepidopteran pests. Background technique [0002] As an important organ of insects, wings endow insects with the ability to fly and increase the space for insects to feed and mate. At present, winged insects have become the focus and difficulty in the control of agricultural pests. Analyzing the molecular mechanism of insect wing development regulation and finding ways to inhibit wing development will provide important theoretical and practical significance for the control of winged insects. [0003] MicroRNA (miRNA) is a kind of small molecule non-coding RNA with a length of about 22nt, which is highly conserved in animal evolution. By recognizing the binding site on the 3'-UTR of the target gene mRNA, miRNA causes the transcriptional repression of the target gene or the degradation of the mRNA, and then re...

Claims

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Application Information

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IPC IPC(8): A01N63/60A01P7/04
CPCA01N63/60
Inventor 周紫章刘庆信赵云贺于璇
Owner SHANDONG AGRICULTURAL UNIVERSITY
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