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Novel trichoderma viride strain and application thereof

A technology of Trichoderma viride and new strains, which is applied in the field of bioengineering, can solve the problems of few species of Trichoderma strains and cannot meet the demand for yield, etc., and achieves the effect of expanding the scope of application

Active Publication Date: 2021-02-26
WULIANGYE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is: the existing 6-pentyl-2H-pyran-2-one-producing Trichoderma strains are few in variety and cannot meet the production demand

Method used

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  • Novel trichoderma viride strain and application thereof
  • Novel trichoderma viride strain and application thereof
  • Novel trichoderma viride strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The screening of embodiment 1 Trichoderma strain

[0040] Collection medium: 2% lactic acid, 1% glucose, 0.5% peptone, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 0.01% chloramphenicol, 2% agar, prepared with distilled water, natural pH, autoclaved at 121°C for 20 minutes .

[0041] PDA agar medium: 1% glucose, 0.5% peptone, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 0.01% chloramphenicol, 2% agar, prepared with distilled water, natural pH, autoclaved at 121°C for 20 minutes.

[0042] Using the gravity sedimentation method, 100 mL of the prepared collection medium was placed in the laboratory of Wuliangye Technology Center, Yibin City, Sichuan, for 6 hours, sealed with sterile gauze, and incubated at a constant temperature of 28°C for 5 days. For filamentous bacteria of the genus Mycobacterium, pick the bacteria from the inoculation loop and place them in the PDA agar medium, number them and purify them by streaking, repeat the puri...

Embodiment 2

[0043] Morphological characteristics and molecular identification of embodiment 2 Trichoderma bacterial strain of the present invention

[0044] 1. Observation of colony and bacterial morphology

[0045] The activated new strain of Trichoderma was inoculated on the PDA medium with an inoculation loop, cultured at 28°C for 3 days, and the morphological characteristics of the colony were observed; the hyphae were picked, stained with lactic acid carbolic acid cotton blue staining solution, and prepared under the microscope Observe the characteristics of the bacterial cells.

[0046] Morphological characteristics of the colony: The colony is round, with white hyphae diverging, and green spores appear in the center. Such as figure 1 shown.

[0047] Thale cell characteristics: conidiophores can be seen, conidiophores have septa, vertical or acute-angle symmetrical branches, produce conidia, conidia are solitary or clustered, round or oval, such as figure 2 shown.

[0048] 2. ...

Embodiment 3

[0050] Embodiment 3 Trichoderma viride new bacterial strain lactic acid tolerance of the present invention

[0051] PDA medium: 1% glucose, 0.5% peptone, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 0.01% chloramphenicol, prepared with distilled water, natural pH, autoclaved at 121°C for 20 minutes.

[0052] Trichoderma viride suspension: add an appropriate amount of sterile water to the activated Trichoderma viride PDA test tube slant medium, and use a sterile inoculation loop to elute the spores to prepare the spore suspension.

[0053] Lactic acid-PDA fermentation medium: prepare PDA medium, pack 11 parts, each 50mL in a 150mL Erlenmeyer flask, 10 parts of which are added with different amounts of lactic acid to adjust the pH, and the other blank control sample is supplemented with the same amount of PDA medium , natural pH; sterilized at 121°C for 20 minutes, cooled, and under sterile conditions, each was inserted with 1mL Trichoderma viride suspension, cu...

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Abstract

The invention belongs to the technical field of bioengineering, and particularly relates to a novel trichoderma viride strain for brewing production environment air and application thereof. Aiming atthe problems that existing trichoderma strains for producing 6-pentyl-2H-pyran-2-one are few in variety and cannot meet the yield requirement, the trichoderma viride capable of metabolizing and producing 6-pentyl-2H-pyran-2-one is provided and preserved in the General Microbiology Center of China Microbial Culture Collection Management Committee with the preservation number of CGMCC No.20254. Thetrichoderma viride has the capacity of producing 6-pentyl-2H-pyran-2-one, the highest lactic acid tolerance concentration is 52.62 g / L, the lowest tolerance pH value is 1.97 mg / L, and the trichodermaviride has the obvious aspergillus flavus inhibition capacity and has the very high application value.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a new strain of Trichoderma viride and its application. Background technique [0002] Trichoderma viride belongs to the genus Trichoderma, the phylum Deuteromycota, and the order Hyphospora. The main mechanisms by which Trichoderma can be used to prevent diseases or inhibit pathogens can be generally classified into five categories, namely antibiotic production, nutrient competition, microparasitism, cell wall decomposing enzymes, and induction of plant resistance. [0003] Some species of Trichoderma have the property of metabolizing 6-pentyl-2H-pyran-2-one, such as Trichoderma harzianum and Trichoderma dark green. 6-Pentyl-2H-pyran-2-one has the aroma of mushroom, blue cheese, coconut or milk, and has dual characteristics of flavor compounds and biological active substances. It is a valuable food additive and biological control agent. But supply is limited....

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P17/06C12P7/22C12P7/16C12P7/04C12G3/02C12R1/885
CPCC12N1/14C12P17/06C12P7/22C12P7/16C12P7/04C12G3/02C12R2001/885C12N1/145Y02E50/10
Inventor 廖勤俭雷学俊安明哲赵东乔宗伟郑佳李杨华王小琴郭艳
Owner WULIANGYE
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