Saccharomyces cerevisiae strain with high yield of cannabigerol and construction method and application thereof

A technology of Saccharomyces cerevisiae strain and cannabinol, which is applied in the field of synthetic biology, can solve the problems of low yield of cannabinol and can not coordinate the synthesis amount, etc., and achieves the effect of increasing the accumulation amount

Pending Publication Date: 2021-02-26
SENRIS BIOTECHNOLOGY (SHENZHEN) CO LTD
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Problems solved by technology

[0003] However, there are several problems in the prior art: first, in Saccharomyces cerevisiae, the synthesis amount of the metabolic precursors GPP and OLA in the synthesis of cannabigerolic acid cannot be coordinated, which eventually leads to the low production of cannabigerlic acid; Second, OLA metabolites cannot be accumulated in large quantities in S. cerevisiae for the synthesis of cannabigerolic acid

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  • Saccharomyces cerevisiae strain with high yield of cannabigerol and construction method and application thereof
  • Saccharomyces cerevisiae strain with high yield of cannabigerol and construction method and application thereof
  • Saccharomyces cerevisiae strain with high yield of cannabigerol and construction method and application thereof

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Embodiment Construction

[0032] In order to make the above objects, features and advantages of the present invention more comprehensible, specific implementations of the present invention will be described in detail below in conjunction with the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. However, the present invention can be implemented in many other ways different from those described here, and those skilled in the art can make similar improvements without departing from the connotation of the present invention, so the present invention is not limited by the specific embodiments disclosed below.

[0033] The invention discloses a Saccharomyces cerevisiae strain with high cannabigerol production. TKS and OAC enzymes are inserted into the Saccharomyces cerevisiae strain A with high cannabigerol production by gene editing technology to obtain Saccharomyces cerevisiae strain B (yG002 st...

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Abstract

The invention discloses a saccharomyces cerevisiae strain with high yield of cannabigerol and a construction method and application thereof. The saccharomyces cerevisiae strain with high yield of cannabigerol is obtained by inserting TKS and OAC enzymes into a saccharomyces cerevisiae strain A capable of producing the cannabigerol through gene editing technology. According to the invention, the gene editing technology is used for regulating and controlling the gene copy numbers of the 2, 4-olivetol synthase TKS and the 2, 4-olivetolic acid synthase OAC, and the accumulation amount of OLA is increased. Finally, the metabolic flux of the cannabigerolic acid synthesis precursors (GPP and OLA) is balanced, and the purpose of high yield of cannabigerolic acid is achieved.

Description

technical field [0001] The invention relates to the technical field of synthetic biology. Through genetic engineering transformation, a recombinant Saccharomyces cerevisiae strain with high production of cannabigerol is obtained, in particular to a Saccharomyces cerevisiae strain with high production of cannabigerol and its construction method and application. Background technique [0002] Cannabigerol (CBG), which has no hallucinogenic side effects, has important application value in the fields of medicine, food, health care and cosmetics. CBG combines with human CB1 and CB2 receptors to perform functions such as anti-tumor, anti-inflammation, anti-oxidation, nerve protection, immune regulation and cancer treatment. At the same time, CBG also has a bactericidal effect on methicillin-resistant Staphylococcus aureus. In the basal cells of the glandular trichomes of the cannabis plant, the polyketide pathway in the vacuoles uses fatty acids to synthesize OLA (Olivetolic acid,...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/19C12N15/90C12P7/22C12R1/865
CPCC12P7/22C12N9/00C12N15/905
Inventor 张云丰罗小舟
Owner SENRIS BIOTECHNOLOGY (SHENZHEN) CO LTD
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