Ralstonia piteri ez-50, its metabolites and its application
An EZ-50, metabolite technology, applied in the application, bacteria, microorganisms and other directions, can solve the problem of unclear microbial strains such as Rhizoctonia solani, to reduce pesticide application, excellent inhibition, and ensure the healthy and sustainable development of agriculture Effect
Active Publication Date: 2022-05-31
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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Problems solved by technology
[0004] However, due to the complexity and variability of microorganisms in nature, as well as the wide variety and large amount of mVOC secreted by them, it is not clear whether there are more effective microbial strains in nature and their metabolic mVOC can inhibit the growth of Rhizoctonia solani
Method used
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Embodiment 1
[0035] a. According to the Gram staining method, the screened bacterial strains are stained to show that the bacterial strains are negative bacteria.
[0037] The bacterium is white, and the surface is smooth and moist, and the edges are neat and viscous.
Embodiment 2
[0041] Volatile organic compounds are solids or liquids with carbon as the basic element, which can be rapidly
[0054] Rhizoctonia solani was inoculated on PDA medium and pre-cultured for 48 hours. Cut the rice leaves into 5 cm long pieces,
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Abstract
The invention discloses a strain of Ralstonia peterii EZ‑50, its metabolites and applications thereof, aiming to solve the technical problem of poor rice sheath blight control effect in the prior art. Ralstonia pickettii (Ralstonia pickettii) CGMCC NO.20622 of the present invention can strongly inhibit Rhizoctonia solani, and can be used to prevent and treat plant diseases caused by Rhizoctonia solani, thereby achieving the goal of reducing pesticide application Purpose, to ensure the sustainable development of agriculture, has broad application prospects; the present invention can metabolize and secrete multiple antibacterial active substances ethyl benzoate, 6-methyl-5-heptene-2 -ketone, 2-ethyl-1-hexanol, dimethyl disulfide, and these volatile substances have a good inhibitory effect on Rhizoctonia solani.
Description
Ralstonia pischei EZ‑50, its metabolites and applications technical field The present invention relates to the technical field of biological control, be specifically related to Ralstonia pickettii) EZ‑50, its metabolites and their applications. Background technique [0002] Rice is an important staple food crop in the world, with more than 3.5 billion people needing rice for energy every day. Yuliku Rice sheath blight caused by Rhizoctonia is one of the most important rice diseases in the world, causing devastating crop losses and serious threaten global food security. At present, the occurrence and development of rice sheath blight are mainly controlled by fungicides in agricultural production. For example, Jinggangmycin has been widely used in China to control sheath blight over the past 30 years. However, the continuous large-scale use of a single Fungicides greatly increase the risk of pathogens developing resistance. And the breeding of resistant varieties i...
Claims
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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/62C12P7/26C12P7/04C12P11/00A01N63/20A01N37/10A01N35/02A01N31/02A01N41/12A01P3/00A01P21/00C12R1/01
CPCC12P7/62C12P7/26C12P7/04C12P11/00A01N63/20A01N37/10A01N35/02A01N31/02A01N41/12C12R2001/01C12N1/205A01P3/00
Inventor 宋阿琳范分良王恩召
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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