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Application of GmAAP protein and GmAAP gene in soybean breeding

A soybean and gene technology, applied in the fields of application, genetic engineering, DNA/RNA fragments, etc., can solve problems such as affecting soybean plant type, and achieve the effect of increasing the number of pods per plant, increasing the number of branches, and increasing the number of branches.

Active Publication Date: 2021-02-26
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the effect of amino acid transporters on soybean plant architecture

Method used

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  • Application of GmAAP protein and GmAAP gene in soybean breeding
  • Application of GmAAP protein and GmAAP gene in soybean breeding
  • Application of GmAAP protein and GmAAP gene in soybean breeding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] Construction of GmAAP Gene Silenced Plants

[0033] Refer to the GmAAP gene information in the soybean reference genome in the soybean genome database (https: / / www.soybase.org) and use the online website (http: / / crispr.hzau.edu.cn / CRISPR2 / ) to design the target of the gene.

[0034] The principles of target design are as follows:

[0035] 1) The knockout site is located in the coding (CDS) region and as far as possible in the front of the protein or in the important functional domain region;

[0036] 2) Try to cover a higher proportion of transcripts;

[0037] 3) There is no off-target or the off-target is located in the intergenic region;

[0038] 4) Optimizing targets with higher editing efficiency;

[0039] 5) The sequence has relatively balanced GC content and is not easy to form secondary structure.

[0040] The target sequence is shown in SEQ ID NO:3:

[0041] gRNA: 5'-ATGTCATATTCCAAGTATCC-3', SEQ ID NO:3.

[0042] The structural diagram of the main function...

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Abstract

The invention belongs to the field of plant genetic engineering, and particularly relates to application of a GmAAP protein and a GmAAP gene in soybean breeding. The invention provides an applicationof a GmAAP protein in soybean breeding, the amino acid sequence of the GmAAP protein is shown as SEQ ID NO: 1. By reducing the expression of the GmAAP gene through a genetic engineering technology, the branches of soybeans can be remarkably increased, so that the yield of the soybeans is increased. It can be seen from the embodiment that the application provided by the invention can effectively increase the branches of soybeans and increase the pod number of a single plant, so that the yield of soybean is increased.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, in particular to the application of GmAAP protein and GmAAP gene in soybean breeding. Background technique [0002] Nitrogen is one of the macroelements necessary for plant growth, and the lack of nitrogen directly affects the yield and quality of crops. In order to ensure the yield and quality of crops, a large amount of nitrogen fertilizer is applied to the soil every year. However, due to the low absorption and utilization rate of nitrogen fertilizer, excessive application of nitrogen fertilizer not only has a very limited contribution to crop yield, but also leads to waste of resources and environmental pollution. question. Therefore, improving the ability of plants to utilize nitrogen is of great significance to the sustainable development of agricultural production. It is an effective way to solve the above problems by using genetic engineering technology to improve the ability of...

Claims

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Application Information

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IPC IPC(8): C12N9/00C12N15/52C12N15/82A01H5/00A01H6/54
CPCC12N9/00C12N15/8261C12N15/8218C07K14/415C12N15/8213Y02A40/146
Inventor 张丹吕海燕杨宇明张恒友王莉
Owner HENAN AGRICULTURAL UNIVERSITY
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