Method for determining content of hydroxysafflor yellow A
A technology of hydroxysafflower and yellow pigment, which is applied in measuring devices, color/spectral characteristic measurement, material analysis through optical means, etc., can solve the problems of time-consuming and expensive, and achieve less time-consuming, simple method, The effect of cheap equipment
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[0044] Preparation of reference substance solution: Take an appropriate amount of analytically pure hydroxysafflower yellow A reference substance, accurately weigh it, add water to make a solution containing 0.1 mg of hydroxysafflower yellow A reference substance per 1 ml, and obtain the reference substance solution.
[0045] The determination method includes the following steps: 10 μl each of the reference substance solution and the safflower injection are precisely drawn, injected into a liquid chromatograph, and the peak area of hydroxysafflower yellow A is measured to obtain the data in Table 1 below.
[0046] Table 1 Statistical table of the total peak area of hydroxy safflower yellow under different detection wavelengths.
[0047]
Embodiment 1
[0049] This example provides a method for determining the content of hydroxysafflower yellow A in safflower injection. It includes the following steps:
[0050] (1) Take the safflower injection to be tested and dilute it 250 times, then use a UV-visible spectrophotometer to perform wavelength scanning, the scanning wavelength range is (200-600nm), the scanning step is 1nm, and the scanning speed is selected as medium speed.
[0051] (2) Obtain and measure the absorbance of the safflower injection to be tested at 217nm, 227nm, 267nm, 292nm, 403nm, 450nm, 480nm and 500nm respectively, and then substitute the above absorbance into the following mathematical model to calculate and obtain the hydroxysafflower yellow pigment A content;
[0052] The mathematical model is:
[0053] Y=-0.114*X1+0.0694*X2-0.0201*X3-0.0907*X4+1.059*X5+0.744*X6-0.110*X7-0.272*X8-0.278*X9-0.043;
[0054] The absorbance of the safflower injection to be tested at 217nm, 227nm, 267nm, 292nm, 403nm, 450nm, ...
Embodiment 2
[0057] This example provides a method for determining the content of hydroxysafflower yellow A in safflower injection. It includes building models and testing models, including:
[0058] (1) Build a model
[0059] The content of hydroxysafflor yellow A in the sample was detected by high performance liquid chromatography as the dependent variable. Specifically, the safflower injection was taken, and its content was detected by high performance liquid chromatography. Samples numbered S1-S10 are safflower injections taken from different samples for modeling; samples numbered CH1-CH20 are taken from safflower injections completely different from S1-S10 and used for subsequent models For inspection.
[0060] The test results are shown in Table 2. Table 2 also shows the content of hydroxysafflower yellow A in another 20 batches of safflower injection after being detected by high performance liquid chromatography.
[0061] Table 2 Detection results of hydroxysafflower yellow A i...
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