Unlock instant, AI-driven research and patent intelligence for your innovation.

High-purity zhongshengmycin F reference substance and preparation method thereof

A technology of shengmycin and reference substance, which is applied in the field of high-purity zhongshengmycin F reference substance and its preparation, can solve the problems of incapable qualitative and quantitative analysis of zhongshengmycin, incapable of qualitative analysis of products, long detection time, etc., and achieves Enhance sustainable development, reduce quality control costs, and solve the effects of uneven quality levels

Pending Publication Date: 2021-03-05
西安麦斯迪生物工程有限公司
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the detection of Zhongshengmycin in China is mostly based on bioassay methods. As we all know, bioassay methods cannot perform effective qualitative analysis on products in the process of quality control, and the detection time is long. At present, even some manufacturers use efficient The liquid phase method is used as a quality control method, but because there is no reference substance for Zhongshengmycin F in the market, none of the methods carried out by the company can carry out effective qualitative and quantitative analysis of Zhongshengmycin F, so purification and preparation of high-purity Zhongshengmycin F It is very important for reference substances to be used in product quality control

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High-purity zhongshengmycin F reference substance and preparation method thereof
  • High-purity zhongshengmycin F reference substance and preparation method thereof
  • High-purity zhongshengmycin F reference substance and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1) Strong-acid cationic resin adsorption separation: Dissolve the Zhongshengmycin master drug and pure water according to the mass ratio of 1:20, let it stand for about 2 hours, take the supernatant and put it on the strong acid cationic resin , the adsorption feed flow rate is 1.0BV / h, after the adsorption is saturated, the resin column is washed with pure water, and then analyzed with 6NaCl solution, and the analyzed solution is collected;

[0034] 2) Activated carbon decolorization: Add 0.3% activated carbon according to the volume of the analysis solution, stir for 30 minutes, control the temperature at 35~37°C, let it stand for 2 hours, filter with filter paper, and remove the activated carbon;

[0035] 3) Concentration under reduced pressure, desalination with methanol: Concentrate the filtrate in step 2) with a rotary evaporator to reduce volume, the concentration pressure is -0.1Mpa, and the temperature is 50°C. During the concentration process, the salt will gr...

Embodiment 2

[0041] 1) Strong-acid cationic resin adsorption separation: Dissolve the Zhongshengmycin master drug and pure water according to the mass ratio of 1:20, let it stand for about 2 hours, take the supernatant and put it on the strong acid cationic resin , the flow rate of the adsorption feed is 1.5BV / h. After the adsorption is saturated, the resin column is washed with pure water, then analyzed with 5% NaCl solution, and the analyzed solution is collected;

[0042] 2) Activated carbon decolorization: Add 0.5% activated carbon according to the volume of the analytical solution, stir for 30 minutes, control the temperature at 35~37°C, let stand for 2 hours, filter with filter paper, and remove the activated carbon;

[0043]3) Concentrate under reduced pressure, desalt with methanol: Concentrate the filtrate in step 2) with a rotary evaporator to reduce volume, the concentration pressure is -0.1Mpa, and the temperature is 55°C. During the concentration process, the salt will gradual...

Embodiment 3

[0049] 1) Strong-acid cationic resin adsorption separation: Dissolve the Zhongshengmycin master drug and pure water according to the mass ratio of 1:20, let it stand for about 2 hours, take the supernatant and put it on the strong acid cationic resin , the flow rate of the adsorption feed is 1.5BV / h. After the adsorption is saturated, the resin column is washed with pure water, then analyzed with 5% NaCl solution, and the analyzed solution is collected;

[0050] 2) Activated carbon decolorization: Add 0.5% activated carbon according to the volume of the analytical solution, stir for 30 minutes, control the temperature at 35~37°C, let stand for 2 hours, filter with filter paper, and remove the activated carbon;

[0051] 3) Concentrate under reduced pressure, desalt with methanol: Concentrate the filtrate in step 2) with a rotary evaporator to reduce volume, the concentration pressure is -0.1Mpa, and the temperature is 55°C. During the concentration process, the salt will gradua...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a high-purity zhongshengmycin F reference substance and a preparation method thereof. The zhongshengmycin F reference substance with the purity of more than or equal to 98% isobtained by sequentially carrying out strongly acidic ion resin adsorption separation, activated carbon decoloration, low-pressure concentration, methanol desalination, desalination gel desalination,low-pressure concentration, high-pressure chromatographic preparation separation, concentration and freeze-drying on a zhongshengmycin technical concentrate. The high-purity zhongshengmycin F reference substance can be applied to quality detection of Zhongshengmycin produced by enterprises at present, fills the blank of Zhongshengmycin reference substances at present, greatly reduces the qualitycontrol cost of Zhongshengmycin, effectively solves the problem of uneven quality levels of Zhongshengmycin, and is beneficial to enhancing the sustainable development of biopesticide enterprises.

Description

technical field [0001] The invention belongs to the technical field of purification of agricultural antibiotics, and in particular relates to a high-purity Zhongshengmycin F reference substance and a preparation method thereof. Background technique [0002] Zhongshengmycin, the chemical name is streptothricins (streptothricins), is one of the earliest discovered antibiotics, among which streptothricin F was discovered by Waksman's research group as early as 1942, but its chemical structure was not until 1982 It was finally determined by artificial total synthesis in 2010. The chemical structure of Zhongshengmycin consists of three parts: streptolactam, gulosamine and lysine side chain. There are 7 main components with bactericidal activity, which are named from 1-7 according to the number of lysine are streptothricins F, E, D, C, B, A and X. [0003] Zhongshengmycin has a broad antibacterial spectrum, can resist Gram-positive bacteria, Gram-negative bacteria, mycobacteria,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/06C07H17/02
CPCC07H1/06C07H17/02
Inventor 张楠杨宏勃潘忠成高波张心心郭秀艳李蒲民
Owner 西安麦斯迪生物工程有限公司