Mesenchymal stem cell storage liquid

Abstract

The invention discloses a mesenchymal stem cell storage liquid, which is an aqueous solution composed of glucose, human serum albumin, a flavone compound, heparin, sodium selenite, fibroblast growth factor and sodium chloride, and specifically comprises: 70-80 g/L glucose, 40-45 g/L human serum albumin, 110-140 [mu]mol/L dihydroquercetin, 0.15-0.2 mol/L heparin, 1.2-1.5 g/L sodium selenite, 0.2-0.4 g/L fibroblast growth factor, and 9 g/L sodium chloride. According to the invention, experiments prove that when the mesenchymal stem cell storage liquid is used for storing mesenchymal stem cells,the survival rate is kept to be 95% or above and can reach 72 hours, the mesenchymal stem cell storage liquid can adapt to long-time and long-distance storage and transportation, the transportation survival rate is high, and the cell activity is not influenced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a mesenchymal stem cell storage solution. Background technique [0002] Mesenchymal stem cells (MSCs) are pluripotent stem cells derived from mesoderm with high self-renewal ability and multi-directional differentiation potential. Separated to get. Studies have confirmed that MSCs have low immunogenicity and are not easy to cause rejection of the body; they have the potential to differentiate into other mesoderm cells and can secrete a variety of cytokines; they have the ability to directional migration. These excellent properties of MSCs make them show good application prospects in damaged tissue repair, organ transplantation, autoimmune disease treatment, tumor treatment and so on. [0003] During the 1st to 8th passages of human mesenchymal stem cells in vitro expansion, MSCs grew rapidly and proliferated exponentially, and the genome of MSCs did not change significa...

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Problems solved by technology

[0004] However, medical institutions that can prepare mesenchymal stem cells must have a GMP (Good Manufacturing Practice) qualified thousand-level cell laminar flow room. After all, such medical institutions are rare, so the wide application of mesenchymal stem cells in clinical practice must solve a problem. The problem of long-distance transportation

At present, there are two commonly used methods of cell transportation. One is low-temperature liquid nitrogen transportation in a non-cultivated state. Cells frozen in liquid nitrogen cannot be transported by plane or high-speed rail. If you choose a car, the liquid nitrogen will be completely volatilized if the transportation time exceeds 12 hours. , the frozen cells will heat up and die, and the activity of frozen cells will decrease

The other is to transport the medium in the culture state. If the transport time exceeds 5 hours, the pH value of the cell culture medium will increase, and the cell morphology will change, which will affect the cell differentiation potential and immune regulation ability, and the risk of cell contamination will increase. raised

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