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Use of kudzu flower extract in prevention and treatment on oxidative damage

A kudzu flower extract and application technology, applied in medical preparations containing active ingredients, food science, drug combination, etc., can solve the problems of limiting oxidative damage disease treatment and prevention, lack of effective treatment of oxidative damage, etc.

Inactive Publication Date: 2021-03-09
益家元品实业(厦门)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Oxidative damage is related to many diseases, such as eye diseases, inflammation, lupus erythematosus, photoaging, psoriasis, etc. However, there is a lack of effective drugs for the treatment of oxidative damage in the existing clinical drugs, which limits the ability to treat oxidative damage. disease treatment and prevention

Method used

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  • Use of kudzu flower extract in prevention and treatment on oxidative damage
  • Use of kudzu flower extract in prevention and treatment on oxidative damage
  • Use of kudzu flower extract in prevention and treatment on oxidative damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Preparation of Pueraria Alcohol Extract

[0095] After Pueraria pudica is crushed, add 5 times by weight of 95% (v / v) ethanol, heat and reflux for extraction for 10 hours, then filter, and the filtrate is concentrated and dried under reduced pressure to obtain a dry extract, i.e. Pueraria kudzu alcohol extract.

Embodiment 2

[0096] Example 2 investigates the improving effect of kudzu flower alcohol extract on oxidative damage

[0097] Pueraria ethanol extract: prepared in Example 1.

[0098] Statistical analysis: M±SD was used for measurement, data analysis was carried out by SPSS software, and t test was used for pairwise comparison, and p<0.05 was considered statistically significant.

[0099] experimental method:

[0100] ARPE-19 cells (human retinal pigment epithelial cells) were seeded in 96-well plates, 1x10 per well 6 Add 100 μL of DME / F12 medium containing 10% FBS to the cells, and after incubation for 24 hours, divide them into blank control group, hydrogen peroxide control group, low-dose group of kudzu flower alcohol extract, medium-dose group of kudzu flower alcohol extract, kudzu flower alcohol extract group, and kudzu flower alcohol extract group. Alcohol extract high dose group, vitamin C group and kudzu flower alcohol extract+vitamin C group, among them, the blank control group a...

Embodiment 3

[0107] Example 3 investigates the inhibitory effect of kudzu flower alcohol extract on the ROS produced by oxidative stress

[0108] Pueraria ethanol extract: prepared in Example 1.

[0109] Statistical analysis: M±SD was used for measurement, data analysis was carried out by SPSS software, and t test was used for pairwise comparison, and p<0.05 was considered statistically significant.

[0110] experimental method:

[0111] ARPE-19 cells (human retinal pigment epithelial cells) were seeded in 96-well plates, 1x10 per well 6 Add 100 μL of DME / F12 medium containing 10% FBS to the cells, and after incubation for 24 hours, divide them into blank control group, hydrogen peroxide control group, low-dose group of kudzu flower alcohol extract, medium-dose group of kudzu flower alcohol extract, kudzu flower alcohol extract group, and kudzu flower alcohol extract group. Alcohol extract high dose group, vitamin C group and kudzu flower alcohol extract+vitamin C group, among them, the ...

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PUM

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Abstract

The invention relates to use of a kudzu flower extract in prevention and treatment on oxidative damage. Specifically, the invention provides use of the kudzu flower extract in preparation of compositions. The compositions are applied to one or more uses as follows: (i) prevention and / or treatment on the oxidative damage; (ii) prevention and / or treatment on oxidative-damage-related diseases; and (iii) uveitis. The kudzu flower extract disclosed by the invention can be used for remarkably inhibiting or reducing active oxygen radicals, thereby being applied to the prevention and / or treatment on the oxidative damage and related diseases thereof such as retinal pigment epithelium lesion and the uveitis.

Description

technical field [0001] The invention relates to the field of medicines, in particular to the use of kudzu flower extracts in preventing and treating oxidative damage. Background technique [0002] Oxidative stress (OS) refers to a state of imbalance between oxidation and anti-oxidation in the body, which tends to oxidize, leading to inflammatory infiltration of neutrophils, increased secretion of proteases, and the production of a large number of oxidative intermediates. Oxidative stress is a negative effect of free radicals in the body and is considered an important factor in aging and disease. [0003] In oxidative damage, reactive oxygen species (ROS) is an important factor causing protein oxidative damage. ROS can be produced through a variety of metabolic pathways, such as chemical poison and drug metabolism, cellular respiration, radiation, light, etc. ROS has a high reactivity. [0004] Oxidative damage is related to many diseases, such as eye diseases, inflammatio...

Claims

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Application Information

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IPC IPC(8): A61K36/488A61P39/06A61P27/02A23L33/105A61K133/00
CPCA61K36/488A61P39/06A61P27/02A23L33/105A23V2002/00
Inventor 叶梓候龙辉王建智
Owner 益家元品实业(厦门)有限公司
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