Unlock instant, AI-driven research and patent intelligence for your innovation.
Enzymolysis polypeptide and application thereof in preparation of anti-leukemia drug
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
An enzymatic hydrolysis and total protein technology, applied in the field of peptides, can solve the problem of very few peptide researches
Active Publication Date: 2021-03-09
魏骏峰
View PDF3 Cites 1 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0004]Currently, the research on Schisandra chinensis mainly focuses on the small molecular compounds in Schisandra chinensis, and there are very few studies on the polypeptides contained in it
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0018] First, experimental materials
[0019] Pulling the dry ripe fruit of North Schisando over 60 mesh sieves, spare.
[0020] Pure water is pure water for Wahaha built; alkaline protease is purchased from Novi.
[0021] Second, the experimental method
[0022] Step S1: Alkali acidic acid extraction total protein
[0023] The dry ripe fruit of northeast is 1 kg first with 10 l of anhydrousethanol heat reflux for 3 h, and the residual solvent is waved. Add 10 L of pure water, adjusting pH to 10.5 with a 10% sodiumhydroxide solution, and stirring at normal temperature for 6 h. The filtrate was extracted to collect the filtrate, then centrifuged at 4000 r / min for 20 min, taking the supernatant, adjusted pH to 4.5 with a 10% hydrochloric acid solution, and centrifuged at 4000 r / min for 20 min, collected, and frozen. protein.
[0038] Take a few long-term JURKAT cell digestion resuspended into a cell suspension, inoculated in a 96-well plate, 1 × 10 per hole 4 Cell, 200 μL, 37 ° C, 5% CO 2 Conditions were cultured for 24 hours, and the complete medium containing 10,20 μg / ml of spicy polypeptide was added, and a pharmace...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Technical field [0001] The present invention belongs to the field of polypeptides, and involves an enzymatic hydrolysis polypeptide and an application, and more particularly to an enzymatic hydrolysis polypeptide and a use of drugs for preparing anti-leukemia. Background technique [0002] Acute lymphocytic leukemia belongs to invasive blood system malignant tumors, accounting for about 75% of leukemia in childhood, and is also common in adults. It is characterized in that the T-progenitorcell abnormally cloned proliferation, resulting in a clinical symptoms such as anemia, anemia, fever, liver spleen, joint pain and systemic lymphatic node, and a large-scale lymph node. Acute lymphocyte leukemia incidence increased year by year, seriously threatening people's lives and health. Finding to develop effective chemotherapeutic drugs is an important way to overcome acute lymphocyte leukemia. [0003] Schisandra is a perennial plantplant for Mulanko, and the mature fruit is dry after...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More 
