Method for promoting rapid nodulation of alfalfa

Abstract

The invention relates to the technical field of biological breeding, in particular to a method for promoting rapid nodulation of alfalfa. The method comprises the following steps of selecting a test strain and an alfalfa variety, preparing a test medium, extracting pantoea agglomerans lipopolysaccharide (LPSp), detecting the activity of the pantoea agglomerans lipopolysaccharide, and carrying outa tissue culture germination test. Germination test seeds are divided to be subjected to two kinds of treatment including disinfection and non-disinfection, a non-disinfection group is not subjected to any treatment, 1 ml, 2 ml and 3 ml of LPSp are added into glass tissue culture germination bottles in a treatment group correspondingly, and the concentrations of the lipopolysaccharide are respectively 0.267 EU / mL, 0.534 EU / mL and 0.801 EU / mL and are marked as A, B and C for treatment; and sterile water treatment is used as a control group. According to the method, 0.267 EU / mL of LPSp promotesnodulation of alfalfa roots of the non-disinfected group, 0.267 EU / mL of LPSp accelerates nodulation of the alfalfa roots of the non-disinfected group, and nodulation is about 18 days earlier than that of the control group; 0.267 EU / mL of LPSp does not affect seed germination including germination potential and germination index; and along with the increase of the concentration of the LPSp, the germination and growth of the alfalfa seeds are inhibited, and the germination and growth of the seeds in the disinfection group are completely inhibited by 0.801 EU / mL of LPSp.

Description

technical field [0001] The invention relates to the technical field of biological propagation, in particular to a method for promoting rapid nodulation of alfalfa. Background technique [0002] Alfalfa ( Medicago sativa L.) is a perennial forage grass belonging to the genus Alfalfa of the family Leguminosae. Its stems and leaves are rich in protein, minerals and vitamins. Because of its rich nutrition, strong adaptability and high grass yield, it has the reputation of "king of grass" , 2011). It can not only be used as a feed crop, but also contribute greatly to improving soil and fertilizing fields, maintaining water and soil, and improving the ecological environment. In addition, alfalfa is the leguminous forage with the largest planting area and the widest planting range in China and even in the world, and it is also the first choice for grassland construction and desertification transformation in western China (Cao Hong et al., 2011). However, because alfalfa is most...

AI Technical Summary

Problems solved by technology

[0003] The symbiotic nitrogen fixation of alfalfa and rhizobia is the basic condition for its high yield (Ma Qidong et al., 1999), but there is bacterial and plant-specific recognition of nitrogen fixation in root nodules (Liu Huiqin, 1994), and it is also affected by many factors (such as soil conditions , The selected rhizobia have poor colonization ability and no nodulation competitive advantage), so screening should be carried out before use to ensure that the rhizobia effectively play a role in nitrogen fixation

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