Legume inoculation agent prepared by using municipal sludge, and preparation method

A technology of municipal sludge and rhizobia, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems that no rhizobia inoculants have been found, so as to promote industrial production and application, reduce production costs, Effect of reducing fermentation cost

Active Publication Date: 2010-09-29
山西卓奇水务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After literature and patent searches, no reports on the production of rhizobia inoculants by sludge fermentation have been found

Method used

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  • Legume inoculation agent prepared by using municipal sludge, and preparation method
  • Legume inoculation agent prepared by using municipal sludge, and preparation method
  • Legume inoculation agent prepared by using municipal sludge, and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, the preparation of fast-growing type soybean rhizobia liquid inoculum

[0029] 1) Municipal sludge sample: The concentrated sludge of a sewage treatment plant in Guangzhou City, Guangdong Province was taken. The basic properties of the sludge are shown in Table 1.

[0030] Table 1. Basic properties of the sludge tested

[0031]

[0032] 2) Sludge medium conditioning: adjust the solid content of the sludge to 3%, add 0.06% foam enemy, and use H 2 SO 4 or NaOH to adjust the pH to 6.5, stir evenly, sterilize in a seed tank or a fermenter at 121°C for 60 minutes under a pressure of 15 lbs, and cool down for later use.

[0033]3) Shake flask culture: pick a ring of rhizobia from the slant culture medium of fast-growing soybean rhizobia ACCC15067 and inoculate it into an Erlenmeyer flask containing a rhizobia liquid culture medium for shake flask culture. The conditions are: temperature 30° C., shaker speed 180 rpm, liquid volume in a 500 mL Erlenmeyer fla...

Embodiment 2

[0038] Embodiment 2, the preparation of fast-growing type soybean rhizobia solid microbial agent:

[0039] 1) municipal sludge sample: same as embodiment 1.

[0040] 2) Conditioning of the sludge medium: adjust the solid content of the sludge to 5%, add 0.5% peanut oil, and use H 2 SO 4 or NaOH to adjust the pH to 7.0, stir evenly, sterilize in a seed tank or a fermenter at 121°C for 30 minutes under a pressure of 15 lbs, and cool down for later use.

[0041] 3) Shake flask culture: pick a ring of rhizobia from the slant culture medium of fast-growing soybean rhizobia ACCC15067 and inoculate it into an Erlenmeyer flask containing a rhizobia liquid culture medium for shake flask culture. The conditions are: the temperature is 29° C., the rotating speed of the shaker is 160 rpm, the volume of liquid in a 500 mL Erlenmeyer flask is 150 mL, and the incubation time is 36 hours. The composition of rhizobia liquid culture medium used is: mannitol 10.0g; Yeast extract 3.0g, KH 2 P...

Embodiment 3

[0046] Embodiment 3, the preparation of rhizobia meliloti solid microbial agent

[0047] 1) Municipal sludge sample: the thickened sludge from a sewage treatment plant in Shenzhen City, Guangdong Province was taken. The basic properties of the sludge are shown in Table 2.

[0048] Table 2. Basic properties of the sludge tested

[0049]

[0050] 2) Conditioning of the sludge medium: adjust the solid content of the sludge to 1.5%, add polypropylene glycol in a ratio of 0.2%, and use H 2 SO 4 Adjust the pH to 6.8 with NaOH, stir evenly, sterilize in a seed tank or a fermenter at 121°C for 50 minutes under a pressure of 15 pounds, and cool down for later use.

[0051] 3) Shake flask culture: a ring of rhizobia was picked from the slant culture medium of Rhizobia meliloti ACCC17512 and inoculated into a Erlenmeyer flask containing a rhizobia liquid medium for shake flask culture. The conditions are: temperature at 28°C, shaker speed at 200 rpm, liquid volume in the Erlenmeyer...

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Abstract

This invention discloses a method for preparing Rhizobium inoculation agent by using city sludge. The method comprises: adjusting the sludge culture medium, culturing by shaking, fermenting in a seed tank, fermenting in a fermentation tank, post-treating the fermentation liquid and examining the product quality. The method can not only treat the sludge, but also obtain Rhizobium inoculation agentproduct with high added values, thus lowering the cost of sludge treatment. The obtained Rhizobium inoculation agent product is liquid or solid, and can effectively accelerate nodulation and nitrogenfixation of leguminous plants. The Rhizobium inoculation agent product can provide nitrogen nutrition for leguminous plants, reduce the application of nitrogen fertilizer, improve the physicochemicalproperties of the soil, and improve the yield and quality of leguminous plants.

Description

technical field [0001] The invention relates to a rhizobia inoculum, in particular to a rhizobia inoculant prepared from urban sludge and a preparation method thereof. Background technique [0002] Leguminous plants-rhizobia symbiotic nitrogen fixation is known as the biological nitrogen fixation system with the strongest nitrogen fixation capacity. The inoculation of rhizobia has become a routine measure to increase the yield of leguminous crops in all countries in the world. There are large-scale production and sales of commercial bacteria in many countries, and the application area is constantly expanding. In my country, legume plants and legume green manure have been inoculated with rhizobia for more than 50 years. Among them, soybeans, peanuts, milk vetch and leguminous pastures have a large inoculation area. It not only has a significant effect on increasing production, but also does not pollute the environment. It is also the earliest microbial fertilizer, the larges...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/41
Inventor 周顺桂王跃强
Owner 山西卓奇水务有限公司
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