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Rhizobium japonicum and application thereof

A technology of Sinorhizobium and soybean, which is applied in the field of microorganisms, can solve the problem of low soybean yield, achieve the effect of promoting soybean nodulation and growth, good effect, and good development and application prospects

Active Publication Date: 2022-05-13
QILU UNIV OF TECH +7
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, my country's arable land resources are limited, and the yield per unit area of ​​soybeans is lower than that of staple crops. In order to ensure that the total grain output does not decline, the expansion of soybean planting area must be completed without competing for land with grain.

Method used

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  • Rhizobium japonicum and application thereof
  • Rhizobium japonicum and application thereof
  • Rhizobium japonicum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1: Capture, isolation, purification and identification of bacterial strain DY23-9

[0066] 1.1 Capture of strains

[0067] 27 samples of saline-alkali soil were obtained from the soybean plot planting test field in the core area of ​​the Yellow River Delta Agricultural High-tech Industry Demonstration Zone in Dongying City, Shandong Province, and the saline-alkali soil layer from the ground surface to 10 cm underground was randomly taken at intervals of 20-30 meters.

[0068] Take soybean seeds with moderate size and complete structure, treat them with 95% ethanol for 1 minute, wash them with sterile water for 2-3 times, treat them with 1% sodium hypochlorite solution for 5 minutes, wash them with sterile water for 5-7 times, place them in 0.7% agar medium at 28°C for 48 hours to accelerate germination After that, the radicle was placed downwards into a plug filled with Dongying soil, and 4 mL of low-nitrogen nutrient solution was applied around the seeds, and t...

Embodiment 2

[0080] Example 2: Physiological function evaluation of bacterial strain DY23-9

[0081] 2.1 Growth curve and generation time of the strain

[0082] Inoculate the strain DY23-9 into YM liquid medium, culture at 28°C and 150r / min, take samples every 8h, measure the OD value of the bacterial liquid with a microplate reader at a wavelength of 600nm, and draw a growth curve. The results are shown in image 3 , The logarithmic growth phase is within 32h, and the OD600 value tends to be stable after 32h, indicating that the growth has entered a stable phase. The generation time of strain DY23-9 calculated from the doubling time of OD600 value in the logarithmic growth phase was 3.0h, and the growth rate was relatively fast.

[0083] 2.2 Stress resistance evaluation of strains

[0084] Based on YMA medium with a pH of 7 at 28°C, set different salt concentrations (0%, 1%, 2%, 3%, 4%, 5% NaCl), different temperatures (14°C, 21°C, 28°C , 35°C, 42°C), different pH (5, 7, 9, 11) and oth...

Embodiment 3

[0086] Example 3: Effects of bacterial strain DY23-9 on nodulation of potted soybeans in saline-alkali soil

[0087] This example was carried out in the Industrial Microbiology Laboratory of the Institute of Biology, Shandong Academy of Sciences. Select soybean seeds with moderate size and complete structure, treat with 95% ethanol for 1 minute, wash with sterile water 2-3 times, treat with 1% sodium hypochlorite solution for 5 minutes, wash with sterile water for 5-7 times, place in 0.7% agar medium at 28°C for 48 hours to accelerate germination , and then implant the radicle down into a tissue culture bottle filled with Dongying saline-alkali soil, apply 10mL low-nitrogen nutrient solution around the seeds, and add 1mL yeast mannitol liquid around the seeds to cultivate the DY23-9 bacterial solution for 3 days. In the blank control treatment, 1 mL of sterile water was added around the seeds, and then the sterile water was poured from time to time to keep the soil moist. Each...

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Abstract

The invention provides rhizobium japonicum and application thereof, and belongs to the technical field of microorganisms. The soybean rhizobium is Sinorhizobium fredii DY23-9, the strain is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms, the preservation date is October 27, 2021, and the preservation number is CGMCC No.23670. The invention further discloses a preparation method of the soybean rhizobium. The strain DY23-9 provided by the invention has the effect of promoting the growth of soybean plants under saline-alkali soil greenhouse potting / field conditions, the promotion effect is embodied in that the nodulation number, the plant height and the stem diameter of soybeans are increased, and the strain DY23-9 has important application value in the aspect of promoting the cultivation and production of soybeans in saline-alkali soil.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a soybean rhizobia strain and its application. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Soybean is an important food and feed crop, providing high-quality oil and protein resources for human beings. With the changes in my country's living standards and dietary structure, the demand for soybean feed in the livestock and poultry industry is increasing year by year. However, my country's arable land resources are limited, and the yield per unit area of ​​soybeans is lower than that of staple crops. In order to ensure that the total grain output does not ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C05G3/00C05G3/80A01G22/40A01G7/06C12R1/01
CPCC12N1/20C05F11/08C05G3/00C05G3/80A01G22/40A01G7/06
Inventor 郑泽慧郭凯王庆玲田志喜任宗明霍毅欣刘书林黄艳华李哲郝永任
Owner QILU UNIV OF TECH
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