Method for obtaining arabian jasmine flower interspecific filial generation through immature embryo rescue technology
A technique for saving young embryos and jasmine flowers, which is applied in the field of obtaining interspecific hybrid progeny of jasmine flowers, and achieves the effects of overcoming obstacles after fertilization, improving the germination rate of young embryos, and improving the germination rate.
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Embodiment 1
[0028] 1. a method for obtaining the interspecific hybrid progeny of jasmine by immature embryo rescue technology, comprising the following steps:
[0029] (1) Artificial cross-pollination: select Jasmine and Jasminum chrysanthemum plants with robust growth, no pests and diseases, and normal development of flower organs as the hybrid female and male parents, and take flowers from the female plants that have grown buds and can open the next day Carry out emasculation and bagging, and after the stigma matures, collect mature pollen from the male plant that blooms on the same day for artificial pollination. The specific operation is: use a clean brush to carefully dip the pollen, and apply it to the emasculated flowers On the stigma, after pollination, continue bagging and isolation, and repeat pollination 3 times.
[0030] (2) Disinfection and pretreatment of immature embryos: Collect capsules 35-40 days after pollination, rinse thoroughly with tap water first, and disinfect wit...
Embodiment 2
[0035] The influence of embodiment 2 different embryonic ages on immature embryo germination
[0036] In order to find out the influence of different embryonic ages on the germination of immature embryos, the capsules were collected 20d, 25d, 30d, 35d, and 40d after cross-pollination, and the immature embryos were stripped and then immature embryo rescue culture was carried out. Each treatment was inoculated with 70 immature embryos. 3 repetitions. After inoculation and culture for 50 days, the germination rate of immature embryos was counted, and the results are shown in Table 1 below. Germination rate of immature embryos (%) = number of germinated embryos / number of inoculated embryos × 100%.
[0037]
[0038] It can be seen from Table 1 that different embryo ages have a great influence on the germination rate of immature embryos, and too small immature embryos can hardly germinate into seedlings. For example, the germination rate of embryo age 20 days is only 7.1%; The ...
Embodiment 3
[0039] The influence of embodiment 3 different pretreatments on immature embryo germination
[0040] In order to ascertain the effect of the pretreatment method of immature embryos on the germination of immature embryos, the immature embryos were taken out and placed in a sterile petri dish with a small amount of sterile water for low-temperature and high-temperature pretreatment before the rescue culture of immature embryos. The normal temperature treatment at 25°C was used as the control, and 80 immature embryos were inoculated for each treatment, repeated three times. The low temperature pretreatment was to treat the immature embryos at 4°C for 2 days; the high temperature pretreatment was to treat the immature embryos at 37°C for 2 days; each group repeated 3 times. Then the immature embryos that had undergone different pretreatments were inoculated into the germination medium for culture, and the germination rate of the immature embryos was counted after 50 days. The resu...
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