Rotary structure of nucleic acid extraction device

Abstract

The invention discloses a rotary structure of a nucleic acid extraction device, relates to the technical field of nucleic acid extraction, and comprises three layers of structures: an upper-layer structure, a middle-layer structure and a lower-layer structure, the upper-layer structure and the middle-layer structure are rotatably connected through a shaft, an elastic element is arranged outside the shaft, the upper-layer structure is provided with a plurality of annularly distributed upper layer liquid storage chambers, the upper-layer liquid storage chambers surround a sample chamber, the bottom of each upper-layer liquid storage chamber is communicated with the bottom of the sample chamber, and the sample chamber extends outwards to form a hole communicated with a reaction chamber; the reaction chamber is located in the middle-layer structure, a groove matched with the shaft is formed in the top of the middle-layer structure, the middle-layer structure is provided with a first channel and a second channel, and the first channel, the second channel and the upper-layer liquid storage chamber are located on the same circle; and the lower-layer structure and the middle-layer structure are coaxially and fixedly connected. Compared with the prior art, the rotary structure of a nucleic acid extraction device has the following beneficial effects: (1) nucleic acid is automatically extracted, which liberates labors, and is convenient and fast; (2) the extraction result is stable; (3) little pollution and high extraction purity are achieved; and (4) the kit is small and exquisite, convenient to carry and operate and wide in application range.

Description

technical field [0001] The present invention relates to the technical field of nucleic acid extraction, in particular to a rotary structure of a nucleic acid extraction device. Background technique [0002] Nucleic acid is the basis of molecular biology research, and high-quality nucleic acid is a necessary prerequisite for molecular markers, gene cloning and gene expression research. Due to the complex composition of biological samples (such as blood, saliva, semen or other secretions), the target nucleic acid in it usually needs to be extracted, purified and amplified before subsequent research can be carried out. The main defects are: the current existing nucleic acid extraction and amplification mainly have the following problems: (1) In the face of huge and complex sample processing, nucleic acid extraction, purification and amplification steps, manual operations are prone to errors, and the overall operation is The steps are complicated, and efficient and rapid target...

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Problems solved by technology

The main defect is that the existing nucleic acid extraction and amplification mainly have the following problems: (1) In the face of huge and complex sample processing, nucleic acid extraction, purification and amplification steps, manual operations are prone to errors, and make the overall operation difficult. The steps are complicated, and efficient and rapid target nucleic acid extraction and amplification cannot be carried out; (2) Most molecular diagnosis needs to be carried out in the laboratory, and many grassroots units do not have the conditions to establish a standard molecular diagnosis laboratory. Due to different operating habits and proficiency, cross-contamination of samples is prone to occur in the process of nucleic acid extraction and amplification; (3) existing nucleic acid extraction instruments and PCR instruments are often large in size and are not suitable for use at the sampling site. To a certain extent, the scope of application of molecular diagnostics is limited

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