Bisphenol A detection method, fluorescence detection kit and application thereof

Abstract

The invention discloses a bisphenol A detection method, a fluorescence detection kit and application thereof. The method is based on an Aptamer, and high-sensitivity BPA detection is realized by virtue of an Aptamer / Catalyst compound and an Initiator 1 / Initiator 2 / Assist compound, a series viscous terminal mediated strand displacement reaction and DNA self-assembly signal amplification strategy and a fluorescence sensitive platform. The method is simple and feasible, low in cost and fast in reaction process. The linear range of detection can be 100fM to 1 micron the effective detection limit can reach 50fM, and similar selectivity can be produced for other possibly existing BPA analogues, so that the method has relatively good precision and accuracy.

Description

technical field [0001] The invention belongs to the field of analysis and detection, and in particular relates to a detection method for bisphenol A, a fluorescence detection kit and applications thereof. Background technique [0002] Bisphenol A (BPA), as an environmental endocrine disrupting chemical, is widely used in the manufacture of food and beverage packaging, baby bottles, thermal paper and other products. Low concentration of BPA will not only pollute the environment, but also endanger human health. The European Food Safety Authority (EFSA) pointed out that the maximum daily intake (TDI) of human BPA should not exceed 4μg / kg. [0003] Currently, conventional BPA detection methods mainly include reversed-phase high-performance liquid chromatography (RP-HPLC), gas chromatography-mass spectrometry (GC-MS), gas chromatography-tandem mass spectrometry (GC-MS / MS) and liquid chromatography. - methods such as tandem mass spectrometry (LC-MS / MS). However, these current m...

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Problems solved by technology

However, these current methods require the separation and enrichment of BPA in the sample, and these methods have the disadvantages of cumbersome operation, time-consuming, and not conducive to rapid on-site detection, which greatly limits the rapid detection of BPA.

[0004] In recent years, the method of using nucleic acid aptamer (aptamer) to detect target has attracted much attention, and analytical techniques such as fluorescence, electrochemical and colorimetry have been established, but most of them have problems such as low sensitivity and difficulty in aptamer screening. Limits the wide application of nucleic acid aptamer-based detection

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