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Application of CUL4B in treatment of type 2 diabetes mellitus

A type 2 diabetes and diabetes technology, applied in the fields of biomedicine and molecular biology, can solve problems such as unclear causes

Inactive Publication Date: 2021-03-19
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cause of metabolic-related diseases such as obesity caused by CUL4B gene mutation has been unclear

Method used

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  • Application of CUL4B in treatment of type 2 diabetes mellitus
  • Application of CUL4B in treatment of type 2 diabetes mellitus
  • Application of CUL4B in treatment of type 2 diabetes mellitus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0168] Materials and methods

[0169] 1. Animal feeding

[0170] The experimental mice were kept in a specific pathogen free (SPF) grade feeding room, kept at a constant temperature of 22°C and alternated day and night for 12 hours each. The mouse high-fat diet was purchased from Research diet, D12492 (60kcal% Fat). Cul4b flox / flox Constructed from this lab, Fabp4-cre + / - Mice were purchased from the Institute of Model Animals, Nanjing University. All mouse experiments in this study were approved by the Animal Ethics Committee of Shandong University School of Medicine.

[0171] To obtain adipose tissue-specific knockout mice, Cul4b flox / flox female mice with Fabp-cre + / - Transgenic mice in which the Cre enzyme was expressed in the adipose system were mated. After mating, the following 2 male mice will be obtained: wild-type male mice (Cul4b flox / Y ) and conditional knockout male mice (Cul4b flox / Y ;Fabp-cre + / - ), the ratio is 1:1.

[0172] 2. Mouse genotype identif...

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Abstract

The invention provides an application of CUL4B in treatment of type 2 diabetes mellitus. Research finds that the CUL4B can negatively regulate adipose differentiation, and further research proves thatCul4b knockout mice can be improved in insulin resistance and type 2 diabetes mellitus symptom. In terms of mechanism, insulin signaling pathways in AKO mice are found to be more sensitive, and adipocytes are found to absorb glucose more easily. Meanwhile, the infiltration of macrophages in adipose tissues of the AKO mice is obviously reduced. Therefore, the CUL4B can be used as a potential drugtarget, the protein activity of the CUL4B can be inhibited to reduce the blood sugar and improve the symptom of the type 2 diabetes mellitus, and the CUL4B has good practical application value.

Description

technical field [0001] The invention belongs to the fields of biomedicine and molecular biology, and specifically relates to the application of CUL4B in the treatment of type 2 diabetes. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] With the rapid development of the economy and the acceleration of urbanization, people's lifestyle and dietary structure have undergone tremendous changes, and the overweight and obese population is increasing year by year. Studies at home and abroad have shown that obesity is an important factor in inducing hypertension, cardiovascular and cerebrovascular diseases, type 2 diabetes, hyperlipidemia, fatty liver and other diseases. A type of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A01K67/027A61P3/04A61P3/10A61P5/48
CPCA01K67/0275A01K2217/20A01K2267/0362A61K45/00A61P3/04A61P3/10A61P5/48
Inventor 李培山宋煜龚瑶琴
Owner SHANDONG UNIV
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