A small molecule inhibitor of polymyxin-resistant protein and its application

A small molecule inhibitor, polymyxin technology, applied in the field of biomedicine, can solve the problem of reducing the efficacy of polymyxin, achieve the effect of inhibiting protein activity and effectively applying space

Inactive Publication Date: 2021-04-27
WUHAN UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Since polymyxin was widely used in animal husbandry and agriculture in the past, there are not many reports of clinical resistance to polymyxin, so polymyxin is considered to be the last line against these "super bacteria" line of defense, however, the presence of MCR-1, especially its highly efficient transfer rate between bacteria, greatly reduces the efficacy of polymyxins

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A small molecule inhibitor of polymyxin-resistant protein and its application
  • A small molecule inhibitor of polymyxin-resistant protein and its application
  • A small molecule inhibitor of polymyxin-resistant protein and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Detect the inhibitory effect of ethanolamine small molecule inhibitors on MCR-1 positive bacteria, comprising the following steps:

[0024] 1) According to the experimental design, first add different concentrations of polymyxin B (polymyxin B) into the MH (Muller Hinton) agar that has been heated and dissolved, and equilibrated in a water bath at 45-50°C, and then the diluted Small molecule inhibitor ethanolamine (ETA) solutions of different concentrations were added to the above-mentioned agar, mixed evenly and poured into a sterilized plate, the thickness of the agar was 3-4 mm;

[0025] 2) Prepare MCR-1-positive bacteria (wide type, WT) and MCR-1-negative bacteria (nagtive cotrol, NC) bacterial suspensions with concentrations equivalent to 0.5 McFarland standard turbidimetric tubes, and serially dilute to 10% of the mother liquor. -2 , 10 -3 ... 10 -7 concentration;

[0026] 3) Inoculate the diluted bacterial solution onto the MH agar plate in step 1) in sequence...

Embodiment 2

[0029] Detecting the inhibitory effect of small molecule inhibitors on MCR-1 protein activity, comprising the following steps:

[0030] 1) Construction of prokaryotic expression plasmid for MCR-1 protein: the gene sequence of MCR-1 was inserted into pET28a plasmid through NcoI and XhoI restriction sites to construct pET28a-MCR plasmid, which was verified by DNA sequencing; MCR-1 The gene sequence is shown in SEQID NO.1;

[0031] 2) Expression of MCR-1 protein: pass the above pET28a-MCR plasmid through CaCl 2 coli Roseta strain, screened by kanamycin and chloramphenicol, the strains grown on LB (Luria-Bertani) culture plates containing kanamycin and chloramphenicol were inoculated into In the LB liquid medium of kanamycin and chloramphenicol, culture at 37°C, 220rpm to the logarithmic growth phase, then add IPTG (isopropylthiogalactopyranoside) to 0.5mM, and induce at 20°C for 20 hours. Finally, the bacteria were collected by centrifugation;

[0032] 3) Purification of MCR-1...

Embodiment 3

[0038] The inhibitory effect of the MCR-1 inhibition site is detected by the method of determining the polymyxin minimum inhibitory concentration of the mutant strain, comprising the following steps:

[0039] 1) Using the pET28a-MCR plasmid obtained in Example 2, 8 possible MCR-1 inhibitory sites: the 246th glutamic acid (E246), the 285th threonine (T285), the 329th day Paragine (N329), Lysine 333 (K333), Histidine 395 (H395), Aspartic acid 465 (D465), Histidine 466 (H466) and 478 Histidine (H478) was subjected to site-directed mutation and mutated into alanine, respectively.

[0040] 2) Pass the above 8 kinds of mutated pET28a-MCR plasmids through CaCl 2 Transformed into Escherichia coli Roseta strain, screened by kanamycin and chloramphenicol, and verified by DNA sequencing.

[0041] 3) Add different concentrations of polymyxin B (polymyxin B) to the MH (Muller Hinton) agar that has been heated and dissolved, and equilibrated in a water bath at 45-50°C, mix well and pour i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
Login to view more

Abstract

The invention belongs to the field of biomedicine, and in particular relates to a small molecule inhibitor of polymyxin drug-resistant protein and application thereof. The present invention discovers for the first time that ethanolamine, as a small molecule inhibitor, can inhibit the drug resistance of polymyxin, inhibit the activity of MCR-1 protein, and inhibit the activity of phosphotransferase similar in structure to MCR-1, and uses ethanolamine as a small molecule inhibitor The combination of drug and polymyxin can make polymyxin play a better role, provide a more effective application space for the application of polymyxin, and has very important applications in the field of pharmaceuticals and animal breeding The value is of great significance for today's widespread drug resistance.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a small molecule inhibitor of polymyxin drug-resistant protein and application thereof. Background technique [0002] Polymyxin was discovered in the 1960s. It is a polypeptide antibiotic against Gram-negative bacteria. It was first discovered in the culture medium of Paenibacillus polymyxa. Polymyxin is mainly composed of a cationic polypeptide ring and a lipophilic fatty acid side chain in structure. There are five types of polymyxins, A, B, C, D, and E, and the three types of A, C, and D are more toxic. The toxicity is less, so B and E are used clinically, and B and E have only one amino acid difference, but they have basically the same biological activity and function. The clinical use of polymyxin is not much, and it is precisely because of this that bacteria have not developed enough resistance to it. Today, when carbapenem antibiotic resistance is becoming stronger ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/133A61K38/12A61P31/04A23K20/195
CPCA61K31/133A61K38/12A23K20/195
Inventor 殷雷魏鹏程宋广济
Owner WUHAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap