Centrifugal micro-fluidic chip and exosome extraction method

A microfluidic chip and extraction method technology, applied in the field of microfluidics, can solve the problems of long time-consuming and cumbersome operation process

Active Publication Date: 2021-03-19
BIOISLAND LAB
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, using the above method to extract exosomes usually requires multiple sample additions

Method used

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  • Centrifugal micro-fluidic chip and exosome extraction method
  • Centrifugal micro-fluidic chip and exosome extraction method
  • Centrifugal micro-fluidic chip and exosome extraction method

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Embodiment Construction

[0034] In order to facilitate the understanding of the present invention, the following will describe the present invention more fully and give preferred embodiments of the present invention. However, the present invention can be embodied in many different forms and is not limited to the embodiments described herein. On the contrary, these embodiments are provided to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0035] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field of the invention. The terms used herein in the description of the present invention are for the purpose of describing specific embodiments only, and are not intended to limit the present invention. As used herein, the term "and / or" includes any and all combinations of one or more of the associated listed items.

[0036] The centrifugal microflui...

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Abstract

The invention relates to a centrifugal micro-fluidic chip and an exosome extraction method. The centrifugal micro-fluidic chip comprises a rotation center and at least one extraction mechanism, and the extraction mechanism comprises a sample injection unit, a capture and release unit and an enrichment unit; the sample injection unit is used for inputting and storing sample liquid; the capturing and releasing unit is connected with the sample injection unit, is positioned at the downstream of the sample injection unit and is used for capturing or releasing an extraction target object in sampleliquid; and the enrichment unit is connected with the capture and release unit, is positioned at the downstream of the capture and release unit and is used for enriching the extracted target object released by the capture and release unit. By utilizing the centrifugal micro-fluidic chip, the purpose of rapidly extracting the exosome can be achieved by only adding a sample once and controlling thechip to rotate, the operation is simple and convenient, the extraction speed is high, the efficiency is high, the reagent consumption is low, and the amount of the extracted exosome is large.

Description

technical field [0001] The invention relates to the field of microfluidic technology, in particular to a centrifugal microfluidic chip and an exosome extraction method. Background technique [0002] Exosomes are extracellular nanoscale vesicles formed by cells through the process of "endocytosis-fusion-efflux". The size is between 50nm and 150nm. They are high in blood and have the ability to transport nucleic acids across the blood-brain barrier ( The properties and functions of biologically active molecules such as miRNA), proteins and lipids. Exosomes, as a carrier for communicating and transferring substances between cells, play a regulatory role in recipient cells, and have been found to play a vital role in a series of physiological and pathological processes. Exosomes are currently extracted mainly from body fluids such as blood, saliva, urine, cerebrospinal fluid, semen, saliva, pleural effusion, and milk. [0003] Exosome extraction methods mainly include ultracen...

Claims

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Application Information

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IPC IPC(8): B01L3/00C12M1/00C12M1/34C12M3/06C12N5/00G01N1/28G01N1/34G01N1/40
CPCB01L3/502707C12M23/16C12M29/04C12M41/42C12N5/00G01N1/286G01N1/34G01N1/4005G01N1/4077G01N2001/4088
Inventor 董鸣王洪赵靓杨子仪
Owner BIOISLAND LAB
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