A kind of tissue culture method using Taibai Fritillaria bulbs as explants

Abstract

The invention provides a tissue culture method using the bulbs of Fritillaria taibai as explants, which solves the difficulties in the supply of provenance in the artificial planting industry of Fritillaria taibai in the prior art, the long production cycle and high production cost, which lead to the artificial planting of Fritillaria taibai. Breeding technology cannot be vigorously developed and cannot meet the technical problems of market supply. In this method, 3-4-year-old bulbs of Fritillaria taibai are used as explants, and the tissue culture and rapid propagation of Fritillaria taibai is finally established through the steps of explant surface sterilization, primary culture, proliferation culture, bud induction culture, and rooting culture. system. The present invention can be widely used in intensive planting of Taibai Fritillaria in greenhouses, shortens the production cycle of Taibai Fritillaria medicinal materials, reduces production costs, better meets market needs, and can also be used to cultivate detoxified Fritillaria seedlings, reducing artificial high Diseases occur in the process of density planting, increase yield, reduce agricultural product input, and reduce costs.

Description

A kind of tissue culture method using the bulbs of Echinacea as explants technical field The present invention relates to the tissue culture method that a kind of fritillary bulb is explant. Background technique [0002] Chuan Fritillaria is a famous authentic medicinal material from Sichuan, and the source of the medicine mainly comes from wild resources, and the contradiction between supply and demand in the market is very sharp. The price is rising year by year, and the price of the best quality Fritillaria is as high as 3600-4000 yuan / kg, which is a typical precious Chinese medicinal material. too Fritillaria Taipaiensis P.Y.Li, as one of the base plants of Sichuan Fritillaria, is mainly distributed in Qinba Mountains It is a characteristic and rare traditional Chinese medicine resource in this area, and it is the only one of the five Sichuan Fritillaria-based species that is suitable for farming at an altitude of about 2000m. The excellent varieties of Fritillari...

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Problems solved by technology

[0003] The object of the present invention is to provide a kind of tissue culture method that takes Taibai Fritillaria bulbs as explants, to solve the difficulty in provenance supply of Taibai Fritillaria artificial planting industry in the prior art, long production cycle, high production cost, resulting in The development of artificial planting industry is slow, and the medicinal materials cannot meet the technical problems of market demand

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