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A kind of Trichoderma multispora and application thereof

A technology of Trichoderma polysporum and Dendrobium officinale is applied in the field of microorganisms to achieve the effects of reducing the incidence rate, wide application prospect and improving disease resistance

Active Publication Date: 2022-05-24
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the use of Trichoderma polyspora to control the disease of Dendrobium officinale plant fungus Delphinium sp.

Method used

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  • A kind of Trichoderma multispora and application thereof
  • A kind of Trichoderma multispora and application thereof
  • A kind of Trichoderma multispora and application thereof

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Experimental program
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Effect test

Embodiment 1

[0014] Embodiment 1: Trichoderma polyspora of the present invention is a bacterial strain that can be obtained from Dendrobium officinale leaves, stems and roots of three-dimensional cultivation in Yiwu City, Zhejiang Province. The Trichoderma polyspora of the present invention is obtained by separating and obtaining according to the following steps: rinse the collected fresh and healthy stems, leaves and roots of Dendrobium officinale under tap water, use sterile filter paper to absorb surface moisture, and cut the roots and stems into 3 cm long The leaves were cut into small pieces of 3cm×3cm, and subjected to three-step surface disinfection treatment with 75% ethanol for 1min→2.5% sodium hypochlorite for 3min→75% ethanol for 30s. After disinfection, the surface of the material was rinsed three times with sterile water and then dried with sterile filter paper. The roots and stems were cut into small pieces (1.0cm) and the leaves were cut into small pieces (0.5cm×0.5cm) with s...

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PUM

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Abstract

The invention discloses a Trichoderma polyspora ( Trichoderma polysporum ) DCR2 strain, deposit unit: China Center for Type Culture Collection, date of deposit: March 24, 2020, deposit number CCTCC M 2020058. The strain was cultured on a potato dextrose agar medium (PDA) plate at 25° C., and the colony was white felt-like and sparse, and the back of the colony was yellow. Trichoderma multispora ( Trichoderma polysporum ) DCR2 can effectively inhibit the growth of the fungus Sclerotium delphinii, significantly reduce the incidence of Dendrobium officinale seedlings, and improve the disease resistance of Dendrobium officinale seedlings. At the same time, it has a certain effect on the prevention and control of various crop diseases caused by the fungus Sclerotium delphinii.

Description

technical field [0001] The invention relates to a Trichoderma polysporum and its application, belonging to the technical field of microorganisms. Background technique [0002] Dendrobium catenatum (D.officinale) is a traditional and precious medicinal material, which has the functions of invigorating the stomach and promoting body fluid, nourishing yin and clearing away heat, and inhibiting tumors. With the continuous increase in the cultivation scale and management intensity of Dendrobium officinale, the harm of harmful organisms has become increasingly serious. Among them, Sclerotium delphinii, which infects the leaves, stem bases and roots of Dendrobium officinale, causes white silkworm disease, which is difficult to prevent and control, and causes serious Economic losses. White silkworm is a common and devastating disease with a wide range of pathogenic bacteria, which can cause serious diseases of more than 500 crops such as peanut, chickpea, tobacco, camellia oleifera...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/38A01P3/00C12R1/885
CPCA01N63/38
Inventor 吴令上斯金平陈秋燕周莹
Owner ZHEJIANG FORESTRY UNIVERSITY