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Compound for promoting pollen germination and application thereof

A technology of pollen germination and compounds, applied in the direction of steroids, applications, chemicals for biological control, etc., can solve problems that need to be studied

Inactive Publication Date: 2021-04-06
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, compounds currently suitable for promoting pollen germination remain to be investigated

Method used

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  • Compound for promoting pollen germination and application thereof
  • Compound for promoting pollen germination and application thereof
  • Compound for promoting pollen germination and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] In order to explore the biological mechanism of PGP1 regulating plant pollen germination, the present invention firstly uses the model plant Arabidopsis thaliana as an experimental material to test the promoting effect of different concentrations of PGP1 on pollen germination. The specific steps are as follows:

[0033](1) Prepare pollen germination medium and distribute it into 1.5mL EP tubes, 1mL per tube (recipe: 0.01% boric acid, 5mM CaCl 2 , 5mM KCl, 1mM MgSO 4 , 18% sucrose). Add 0.012-0.014g of low-melting-point agarose to 1mL of liquid medium; put the EP tube in hot water until the liquid is clear (in order to fully melt, you can change the hot water once in the middle); draw 60-80μL of liquid, evenly (If it is necessary to add PGP1, when the culture medium is cooled to a suitable temperature, add the PGP1 solution of the required concentration, mix well, and then add it to the glass bottom dish); place it at room temperature for 4-8 minutes, etc. After the cu...

Embodiment 2

[0042] In this example, the stored pollen of early-maturing, mid-maturing, and late-maturing varieties of pear trees were used as experimental materials, and the promotion of different concentrations of PGP1 on the germination of stored pollen was tested. The specific steps are as follows:

[0043] (1) After the pollen is collected, wrap it with a layer of sulfuric acid paper, store it in a storage box, put silica gel in the storage box, and store it in a -20°C refrigerator for a long time in a low-temperature, dry place.

[0044] (2) After one year of storage, take out the storage box for storing pollen from the -20°C refrigerator, put it in a 4°C refrigerator for 6-10 hours, and then place it in a 25°C room temperature for 2-4 hours.

[0045] (3) Prepare liquid medium A (100mL): weigh 10g sucrose, 0.03g calcium nitrate, 0.01g boric acid, 0.5856g MES, 10g PEG in a 100mL beaker, dissolve with double distilled water (RO water), and place the measuring cylinder on the magnetic fo...

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Abstract

The invention discloses a compound for promoting pollen germination and application thereof. The compound has a structure shown as a formula I, wherein X is -C(O)- or -(R9)C(R10)-; R1, R2, R3, R4, R5, R6, R7, R8, R9 and R10 are respectively and independently selected from H, alkyl, alkoxy, alkylamino, halogenated alkyl, hydroxyalkyl, amino, hydroxyl, halogen and ester group. The compound shown in the formula I can effectively promote the germination of plant pollen, and is beneficial to improving the pollination efficiency of plants. Meanwhile, the pollen germination promoter containing the compound shown in the formula I has reversibility and concentration adjustability, can be easily expanded from model plants to commercial crops, provides a scientific theoretical basis for agricultural production, has a potential practical value, and is expected to develop a novel reagent for promoting crop pollination efficiency on the basis of scientific research.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a use of a compound in promoting pollen germination and a method for promoting pollen germination. Background technique [0002] Pollen germination to produce pollen tubes is one of the key processes in plant sexual reproduction. Previous studies have found that mature pollen grains need to be adhered, hydrated, form pollen tubes, and guide to ovules, and release sperm through the explosion of pollen tubes to complete the plant fertilization process (Williams, J.H., Reese, J.B. (2019). Current Topics in Developmental Biology 131, 299-336). The participation of mature pollen in the fertilization process is regulated step by step by multiple genes, such as NPG1, ROPs, RALFs, etc. involved in pollen germination and subsequent steps (Ge, Z., et al. (2017). Arabidopsis pollen tube integrity and sperm release are regulated by RALF -mediated signaling.Science 358,1596-1600);...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J1/00A01N45/00A01H1/02A01G7/06A01P21/00
CPCA01G7/06A01H1/02A01N45/00C07J1/0011C07J1/0014
Inventor 瞿礼嘉李瑞熙吴俊张立王志娟李甲明
Owner PEKING UNIV
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