Rapid cultivation method for transgenic maize inbred line with nitrogen efficiently utilized

Abstract

The invention discloses a rapid cultivation method for the transgenic maize inbred line with nitrogen efficiently utilized, which comprises the following steps of: evaluating the nitrogen efficient utilization efficiency of a first filial generation under the nitrogen stress condition of a nutrient pool, selecting a transformant with optimal nitrogen utilization efficiency and a hybridized single plant of the transformant, and carrying out backcross transformation and inbred line cultivation in a targeted manner. By evaluating the efficient utilization efficiency of transformant nitrogen in advance, low-efficiency transformant materials and backcross progenies thereof are eliminated, the workload is greatly reduced, meanwhile, the planting scale of the selected transformant materials can be enlarged, and follow-up selection is facilitated. Nitrogen stress, large population and phenotype-genotype association selection are adopted, assignment is carried out according to the similarity degree of a certain character and recurrent parent characters, assignment accumulation is carried out on multiple characters, phenotype-genotype association selection is carried out on a single plant according to the integration degree, and homozygous selection of gene loci is carried out after continuous 3-4 representative comprehensive identification. Backcross progenies with the background recovery rate of 99% or above are obtained two years ahead of time.

Description

technical field [0001] The invention belongs to the field of genetic breeding, and in particular relates to a method for rapidly cultivating transgenic maize inbred lines with high nitrogen utilization. Background technique [0002] Maize is a high-yielding crop and occupies an important strategic position in my country's and even the world's grain production. However, in recent years, in order to ensure the high and stable yield of corn, my country has used a large amount of chemical fertilizers in corn production, which has caused ecological and environmental problems such as soil compaction and acidification, eutrophication of water bodies, and increased acid rain. Improving the nitrogen use efficiency of corn can effectively reduce the amount of fertilizer application, reduce environmental pollution, and reduce production costs. The existing nitrogen-efficient maize germplasm resources in my country are extremely scarce, and the cultivation of new nitrogen-efficient tra...

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is: how to solve the time-consuming and labor-intensive problem of returning the existing nitrogen efficient use transgenic receptor to the backbone line to be consistent with the genetic background

Images