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Method for establishing healthy SD male rat liver fibrosis by adopting complex factor method

A liver fibrosis and complex factor technology, applied in biochemical equipment and methods, cell dissociation methods, pharmaceutical formulations, etc., can solve the problems of short time consumption and difficult operation

Active Publication Date: 2021-04-09
昕慕(上海)科技发展有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0009] Aiming at the deficiencies in the prior art, the present invention provides a method for establishing healthy SD male rat liver fibrosis using a compound factor method, which is easy to operate and takes a short time Advantages, solve the problem of difficult operation

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  • Method for establishing healthy SD male rat liver fibrosis by adopting complex factor method

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Embodiment Construction

[0034]Next, the technical solutions in the embodiments of the present invention will be apparent from the embodiment of the present invention, and it is clearly described, and it is understood that the described embodiments are merely embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, there are all other embodiments obtained without making creative labor without making creative labor premises.

[0035]Seefigure 1A method of establishing a health SD male rat liver fibrosis using a composite factor method, including the following steps:

[0036]S1: Cultivation of blood stem cells

[0037]Add PBS dilution by intestinal blood, add 10 ml of lymphocyte isolation, slowly add diluted intestinal blood, slowly absorb the layered middle white cloud-like lymphocyte layer, add PBS, centrifuged for 5 minutes, centrifugation Abandon the discard, add the fetal bovine serum again, mix well, repeat the centrifuge again; discard the supernatant,...

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Abstract

The invention relates to the technical field of medical animal cultivation, and discloses a method for establishing healthy SD male rat liver fibrosis by adopting a complex factor method. The method comprises the following steps of S1, culturing blood stem cells; S2, preparing stem cell active matter, and mixing A, B and schistosome blood cells according to a ratio of 1: 1: 1 to obtain blood stem cell active matter; S3, feeding and culturing adult SD male rats; and S4, feeding the rats for one week, starting to feed the rats with high-fat CDAA feed after the weight of the rats is measured, and injecting the blood stem cell active matter. The blood stem cells, the stem cell active matter and schistosome are mixed to form compound factors, the compound factors are injected into the healthy SD male rats, an animal model prepared from the rats conforms to the pathological process of conversion from NASH to HCC, operation is easy, consumed time is short, stability and effectiveness are achieved, modeling and technical support are provided for development of new drugs for treating non-alcoholic steatohepatitis, and huge economic and social benefits are realized.

Description

Technical field[0001]The present invention relates to the field of medical animal culture, in particular a method of establishing a healthy SD male rat liver fibrosis using a composite factor method.Background technique[0002]Hepatic fibrosis is a pathophysiological process refers to abnormal hyperplasia from intrahemorrheistronis of various pathogenic factors. Any liver damage has a liver fibrosis in the process of liver repair healing. If the damage factor cannot be removed, the process of fibrosis will continue to develop in a long time. Therefore, it is not an independent disease; the composite factor is a general repree of multiple functions or sources related factors.[0003]Currently used to study Nash, hepatic fibrosis, cirrhosis, and HCC model animals mainly chemically induced, physical damage, pathological diet, gene knockout, etc. These technologies have the following problems:[0004](1) Chemical drug induction is to cause DEN or tetrachloride (CCL4) through digestive tract, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02C12N5/0789A61K35/28
CPCA01K67/02C12N5/0647A61K35/28C12N2509/00Y02A50/30
Inventor 杨振亚
Owner 昕慕(上海)科技发展有限公司