Self-healing temperature-sensitive hydrogel and preparation method thereof
A temperature-sensitive hydrogel and self-healing technology, which can be used in pharmaceutical formulations, medical preparations of non-active ingredients, aerosol delivery, etc., can solve the problems of hydrogels that are difficult to repair, and achieve high adhesion and good bioavailability Effect of degradability and low cytotoxicity
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example 1
[0013] Weigh 0.5 g of hexanoylated ethylene glycol chitosan and disperse it into 50 ml of deionized water, place it on a magnetic stirrer and stir at room temperature to fully dissolve, and adjust the pH value to 4.5 with hydrochloric acid. 30 mg of 3,4-dihydroxyphenylpropionic acid and 40 mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride were added to the above solution and the pH was adjusted with sodium hydroxide Adjusted to 5.0, the process is mainly to activate the carboxylic acid of 3,4-dihydroxyphenylpropionic acid for further reaction with the amino group of hexanoylated ethylene glycol chitosan. Then, the reaction was carried out under stirring under nitrogen gas at room temperature for 4 hours. Then pour the obtained reaction solution into a dialysis bag and dialyze with pH 5.0 water for two days, then dialyze with PBS buffer solution for 4 hours, and finally dialyze with water for one day, and freeze-dry the solution after dialyzing to obtain modifie...
example 2
[0015] Weigh 0.5 g of hexanoylated ethylene glycol chitosan and disperse it into 50 ml of deionized water, place it on a magnetic stirrer and stir at room temperature to fully dissolve, and adjust the pH value to 5.0 with hydrochloric acid. 40 mg of 3,4-dihydroxyphenylpropionic acid and 50 mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride were added to the above solution and the pH was adjusted with sodium hydroxide Adjusted to 5.0, the process is mainly to activate the carboxylic acid of 3,4-dihydroxyphenylpropionic acid for further reaction with the amino group of hexanoylated ethylene glycol chitosan. Then, the reaction was carried out under stirring under nitrogen gas at room temperature for 8 hours. Then pour the obtained reaction solution into a dialysis bag and dialyze with pH 5.0 water for two days, then dialyze with PBS buffer solution for 4 hours, and finally dialyze with water for one day, and freeze-dry the solution after dialyzing to obtain modifie...
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