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Process for extracting lipoxygenase in soybean whey protein wastewater by using two aqueous phases

A technology of soybean whey protein and lipoxygenase, which is applied in the extraction of water/sewage treatment, oxidoreductase, enzymes, etc., to achieve high-value utilization, reduce COD and BOD, reduce resource waste and environmental pollution.

Inactive Publication Date: 2021-04-09
QILU UNIV OF TECH
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  • Abstract
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  • Application Information

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Problems solved by technology

[0004] The present invention proposes a novel process for extracting lipoxygenase in soybean whey protein wastewater aiming at the problems existing in the extraction of lipoxygenase in traditional soybean whey protein wastewater

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  • Process for extracting lipoxygenase in soybean whey protein wastewater by using two aqueous phases
  • Process for extracting lipoxygenase in soybean whey protein wastewater by using two aqueous phases
  • Process for extracting lipoxygenase in soybean whey protein wastewater by using two aqueous phases

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Embodiment 1

[0029] Example 1, this example provides specific process steps for two-phase extraction of lipoxygenase in soybean whey protein wastewater.

[0030] First, 500ml of soybean whey wastewater was taken and refrigerated and centrifuged at 8000r / min at 4°C for 10min, and the supernatant was stored at 4°C for later use. Prepare PEG6000 mass fraction 19.2%, (NH 4 ) 2 SO 4 Mixed solution (acidic) with a mass fraction of 10.5% and 20.0% waste water addition, adjust the pH to 6.0 with sodium hydroxide, construct a two-phase aqueous system (the total system is 10g), mix well, and then stand for 3h, (NH 4 ) 2 SO 4 The layer was centrifuged at 12000r / min at 4°C for 5min, and the separated LOX was obtained from the precipitate.

[0031] After testing, the specific enzyme activity of LOX was 4027.68U / mg, and the recovery rate and purity of the enzyme activity were 89.76% and 28.74%, respectively.

[0032] In addition, after the two-phase extract was concentrated 10 times through a 30KD...

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Abstract

The invention belongs to the technical field of resource utilization of soybean whey wastewater and relates to a process for extracting lipoxygenase in soybean whey protein wastewater by using two aqueous phases. A PEG6000-(NH4)2SO4 two aqueous phase system is adopted for extraction. The invention provides the PEG6000-(NH4)2SO4 two aqueous phase extraction system for separating and extracting LOX in the soybean whey wastewater, provides optimal extraction conditions and combines an optimization model for mutual verification. The process provided by the invention adopts a two aqueous phase extraction separation technology for separating the LOX in the soybean whey wastewater, can reduce COD and BOD in the wastewater, reduce resource waste and environmental pollution and realize high-value utilization of the soybean whey wastewater.

Description

technical field [0001] The invention belongs to the technical field of resource utilization of soybean whey wastewater, and relates to a process for extracting lipoxygenase in soybean whey protein wastewater by two aqueous phases. Background technique [0002] Soybean whey wastewater is a by-product produced by the production of SPI from low-temperature defatted soybean meal by alkali-soluble acid precipitation process. Statistics show that producing 1t of SPI will produce 35-65t of soybean whey wastewater, and soybean whey wastewater is rich in Nutrients, and the pH value is acidic and easily corrupted, causing serious pollution. At present, the main treatment methods of soybean whey wastewater are anaerobic method and aerobic method to reduce the COD value and BOD value in the wastewater, so as to meet the national wastewater discharge standard, but the high treatment cost seriously reduces the production efficiency and Economic benefits, and a large number of active subs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C02F1/26G16B40/00G16B45/00
CPCC02F1/26C12N9/0069C12Y113/11012G16B40/00G16B45/00
Inventor 黄姗张志国褚衍强姜闪赵国建赵硕
Owner QILU UNIV OF TECH