Swertia bimaculata extract and application thereof in preparation of antidepressant drugs

Abstract

The invention discloses a swertia bimaculata extract. A preparation method of the swertia bimaculata extract comprises the following steps: S1, drying in the shade, chopping or crushing; S2, extraction; S3, concentrating, degreasing, column chromatography separation, and collection of an eluent; and S4, concentrating, evaporation of an organic solvent to dryness, drying and crushing to obtain the swertia bimaculata extract. In-vitro pharmacodynamic experiment results show that the swertia bimaculata extract provided by the invention has a very good protection effect on SH-SY5Y cells, the cell survival rate reaches 84.7%, it is proved that the swertia bimaculata extract has a remarkable anti-depression effect, and animal experiment results show that the swertia bimaculata extract provided by the invention can obviously relieve the behavior desperate state of mice, reduce the suspension immobilization frequency and shorten the tail suspension immobilization time, and has obvious effects on relieving depression emotion. The swertia bimaculata extract provided by the invention has very high anti-depression activity and can be applied to preparation of antidepressant drugs.

Description

technical field

[0001] The invention relates to the technical field of biomedicine, in particular to an extract of Swert sprouts and its application in the preparation of antidepressant drugs. Background technique

[0002] Swert sprouts medicinal plant, Tibetan name "Dida", is an annual dwarf herbaceous medicinal plant of Gentianaceae. It is recorded in the Tibetan medical book "Si Bu Yi Dian" that it has the effects of clearing heat, detoxifying, clearing liver and gallbladder. Modern pharmacology has proved that Yin Chen has various pharmacological activities, including anti-liver damage, anti-depression, anti-tuberculosis, anti-tumor, anti-jaundice, anti-mutation, anti-inflammation, anti-virus, anti-oxidation, diuretic, hypoglycemic and cardiotonic effects. Swert sprouts can also promote the movement of the small intestine; regulate endocrine or affect sexual function; promote hair growth and improve skin function; can metabolize human intestinal bacteria;

[0003] Depre...

Examples

Embodiment 1

[0034] Dry the medicinal material of Swertia chinensis in the shade and chop it to obtain Swertia cunduan; accurately weigh 10 g of Swertia cunduan and add it to 100 mL of ethanol (volume concentration 70%) for immersion for 4 h. Reflux for 4 h, reflux for 3 times in total, and then combine the extracts; concentrate the extracts to a concentration of 1.5 g / mL drug solution, then degrease the concentrated solution with petroleum ether, and then use macroporous adsorption resin for column extraction. For chromatographic separation, choose the wet method to load the column. When elution is performed, wash 1~6 BV column volume with water to remove impurities, and then elute 1~12 BV column volume with ethanol solution with a volume concentration of 30~90%. Dehydration: Concentrate the eluent, evaporate the organic solvent to dryness, dry and pulverize to obtain a total of 1.1 g of Swert sprout extract.

Embodiment 2

[0036] Dry the medicinal materials of Swertia chinensis in the shade and crush them to obtain the coarse powder of Swertia sprouts (40-60 meshes); accurately weigh 10 g of Swerts sprouts coarse powder and add it to 100 mL of ethanol (volume concentration 70%) to soak for 6 h. After the end, reflux the impregnation solution at 100 °C for 2 h, reflux for 3 times in total, and then combine the extracts; concentrate the extracts to a concentration of 1.0 g / mL drug solution, and then degrease the concentrated solution with ethyl acetate , and then use silica gel for column chromatography separation, select the dry method to put on the column, and successively elute with petroleum ether, dichloromethane, ethyl acetate, acetone, ethanol and water, and collect dichloromethane, ethyl acetate, acetone eluted fraction The eluate; the eluate was concentrated, evaporated to dryness of the organic solvent, dried and pulverized to obtain a total of 2.3 g of swert sprout extract.

Embodiment 3

[0038] Dry the medicinal materials of Swertia chinensis in the shade and crush them to obtain coarse Swert sprouts powder (40-60 meshes); Accurately weigh 10 g of Swerts sprouts coarse powder and add it to 100 mL of ethanol (volume concentration 70%) for 2 h. After the end, reflux the impregnation solution at 120°C for 1 h, reflux for a total of 3 times, and then combine the extract solution; concentrate the extract solution to a concentration of 0.5 g / mL drug solution, and then use ethyl acetate to degrease the concentrated solution , and then use macroporous adsorption resin for column chromatography separation, choose wet column loading, wash 1~6 BV column volume with water to remove impurities, and then elute with ethanol solution with a volume concentration of 30~90% 1 to 12 BV column volume, collect the eluate; concentrate the eluate, evaporate the organic solvent, dry and pulverize to obtain a total of 0.68 g of Swert sprout extract.

technical field

[0001] The invention relates to the technical field of biomedicine, in particular to an extract of Swert sprouts and its application in the preparation of antidepressant drugs. Background technique

[0002] Swert sprouts medicinal plant, Tibetan name "Dida", is an annual dwarf herbaceous medicinal plant of Gentianaceae. It is recorded in the Tibetan medical book "Si Bu Yi Dian" that it has the effects of clearing heat, detoxifying, clearing liver and gallbladder. Modern pharmacology has proved that Yin Chen has various pharmacological activities, including anti-liver damage, anti-depression, anti-tuberculosis, anti-tumor, anti-jaundice, anti-mutation, anti-inflammation, anti-virus, anti-oxidation, diuretic, hypoglycemic and cardiotonic effects. Swert sprouts can also promote the movement of the small intestine; regulate endocrine or affect sexual function; promote hair growth and improve skin function; can metabolize human intestinal bacteria;

[0003] Depre...

Examples

Embodiment 1

[0034] Dry the medicinal material of Swertia chinensis in the shade and chop it to obtain Swertia cunduan; accurately weigh 10 g of Swertia cunduan and add it to 100 mL of ethanol (volume concentration 70%) for immersion for 4 h. Reflux for 4 h, reflux for 3 times in total, and then combine the extracts; concentrate the extracts to a concentration of 1.5 g / mL drug solution, then degrease the concentrated solution with petroleum ether, and then use macroporous adsorption resin for column extraction. For chromatographic separation, choose the wet method to load the column. When elution is performed, wash 1~6 BV column volume with water to remove impurities, and then elute 1~12 BV column volume with ethanol solution with a volume concentration of 30~90%. Dehydration: Concentrate the eluent, evaporate the organic solvent to dryness, dry and pulverize to obtain a total of 1.1 g of Swert sprout extract.

Embodiment 2

[0036] Dry the medicinal materials of Swertia chinensis in the shade and crush them to obtain the coarse powder of Swertia sprouts (40-60 meshes); accurately weigh 10 g of Swerts sprouts coarse powder and add it to 100 mL of ethanol (volume concentration 70%) to soak for 6 h. After the end, reflux the impregnation solution at 100 °C for 2 h, reflux for 3 times in total, and then combine the extracts; concentrate the extracts to a concentration of 1.0 g / mL drug solution, and then degrease the concentrated solution with ethyl acetate , and then use silica gel for column chromatography separation, select the dry method to put on the column, and successively elute with petroleum ether, dichloromethane, ethyl acetate, acetone, ethanol and water, and collect dichloromethane, ethyl acetate, acetone eluted fraction The eluate; the eluate was concentrated, evaporated to dryness of the organic solvent, dried and pulverized to obtain a total of 2.3 g of swert sprout extract.

Embodiment 3

[0038] Dry the medicinal materials of Swertia chinensis in the shade and crush them to obtain coarse Swert sprouts powder (40-60 meshes); Accurately weigh 10 g of Swerts sprouts coarse powder and add it to 100 mL of ethanol (volume concentration 70%) for 2 h. After the end, reflux the impregnation solution at 120°C for 1 h, reflux for a total of 3 times, and then combine the extract solution; concentrate the extract solution to a concentration of 0.5 g / mL drug solution, and then use ethyl acetate to degrease the concentrated solution , and then use macroporous adsorption resin for column chromatography separation, choose wet column loading, wash 1~6 BV column volume with water to remove impurities, and then elute with ethanol solution with a volume concentration of 30~90% 1 to 12 BV column volume, collect the eluate; concentrate the eluate, evaporate the organic solvent, dry and pulverize to obtain a total of 0.68 g of Swert sprout extract.

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