Composite strain and application thereof to kitchen waste degradation

A technology of composite bacteria and kitchen waste, applied in the direction of bacteria, climate change adaptation, and microbial-based methods, can solve the problems of complex production process and high cost, and achieve the effects of low energy consumption, small investment, and low storage cost

Active Publication Date: 2021-04-27
杭州鹏创环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the disadvantages of complex production process, high cost, and on-site use of live bacterial preparations for food waste treatment, the invention provides a composite bacterial strain and its Application in degrading kitchen waste. The composite strain has a good effect on kitchen waste and can reproduce normally under high temperature treatment. The bacterial agent made from the composite strain can be preserved for more than 2 years, which is easy to store and manage. Easy to use, ready to use

Method used

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  • Composite strain and application thereof to kitchen waste degradation
  • Composite strain and application thereof to kitchen waste degradation

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Effect test

Embodiment 1

[0046] Embodiment 1 Composite strain of the present invention

[0047] Bacillus subtilis, Bacillus thaliana, Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus tequila were respectively inoculated into the activation culture solution (tryptone 10g / L, yeast extract 5g / L, Sodium chloride 10g / L) in the shaking flask, horizontal vibration 120 turns per minute, 45 degrees centigrade culture 24~48 hours; Obtain the bacterium liquid of each strain activation, the bacterium liquid after the activation is mixed according to the following mass percentage: Said composite strains include 65.5% Bacillus subtilis, 1% Bacillus thaliana, 19% Bacillus amyloliquefaciens, 5% Bacillus licheniformis and 9.5% Bacillus tequila, to obtain the composite strains of the present invention.

Embodiment 2

[0048] Embodiment 2 Composite strains of the present invention

[0049] Inoculate Bacillus subtilis, Bacillus thaliana, Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus tequila into the activation medium (tryptone 10g / L, yeast extract 5g / L, Sodium chloride 10g / L) shake flask, shake horizontally 120 revolutions per minute, 45 degrees centigrade culture 24~48 hours; Obtain the bacterium liquid of each strain activation, the bacterium liquid after activation is mixed according to the following mass percentage: 62 % Bacillus subtilis, 1% Bacillus thaliana, 18% Bacillus amyloliquefaciens, 10% Bacillus licheniformis and 9% Bacillus tequila, to obtain the composite bacterial species of the present invention.

Embodiment 3

[0050] Embodiment 3 Composite strains of the present invention

[0051] Inoculate Bacillus subtilis, Bacillus thaliana, Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus tequila into the activation culture solution (tryptone 10g / L, yeast extract 5g / L, In the shaking flask of sodium chloride 10g / L), shake horizontally at 120 revolutions per minute, and cultivate at 45 degrees Celsius for 24 to 48 hours; obtain the activated bacterial liquid of various strains, and mix the activated bacterial liquid according to the following mass percentage: 51.5 % Bacillus subtilis, 1% Bacillus thaliana, 15% Bacillus amyloliquefaciens, 25% Bacillus licheniformis and 7.5% Bacillus tequila, to obtain the compound bacterial species of the present invention.

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Abstract

The invention relates to the technical field of garbage treatment, in particular to a composite strain and an application thereof to kitchen waste degradation. The composite strain provided by the invention comprises bacillus subtilis, bacillus thuringiensis, bacillus amyloliquefaciens, bacillus licheniformis and bacillus tequilensis. All the strains are reasonably compounded and have a synergistic effect, organic substances such as starch, protein and grease in the kitchen waste can be effectively degraded, the fermentation period is short, and fermentation can be generally completed within 24-48 hours.

Description

technical field [0001] The invention relates to the technical field of garbage treatment, in particular to a composite bacterial strain and its application in degrading kitchen garbage. Background technique [0002] A wide variety of garbage is produced in daily life, and in the past, it was buried or incinerated to reduce the amount and then bury it. With the increase of the population, resources are gradually strained, and the magnetic attraction effect of the city leads to dense population living, resulting in a large amount of waste, occupying the already tight living space, and becoming a key to urban governance. If waste can be turned into treasure, it will increase the available resources and reduce the occupation of land resources, which is a double effect. Therefore, recycling garbage as much as possible has become the object of a lot of people's attention and efforts. Among them, food waste contains a large amount of organic matter, has unpleasant characteristics...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F9/04C05F17/20C12R1/125C12R1/07C12R1/10C12R1/01
CPCC12N1/20C05F9/04C05F17/20Y02W30/40Y02A40/20
Inventor 陈其江
Owner 杭州鹏创环保科技有限公司
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