In-vitro detection system for intestinal migration compound motion and application thereof

A detection system and composite motion technology, applied in the field of neuroscience, can solve problems such as inconvenience of operation, loss of reagents, tissue damage, etc., and achieve the effects of prolonging survival time, accurate motion waveform, and high success rate

Pending Publication Date: 2021-04-27
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the in vivo method, the intestinal tract is usually pulled out from the body and fixed, and the reagent is directly added dropwise to the serosa layer of the intestinal tract, and the body temperature and nutrient supply of the intestinal tract are maintained by using the animal's own blood circulation. Exposure may cause tissue damage and prevent normal movement; there is also a method of in vivo testing using water-isolated and constant-temperature heating of the groove containing intestinal tissue. The disadvantage is that it is inconvenient to operate, and the fixed length, height and position of the small intestine in the groove are difficult to adjust , cannot effectively remove the interference of breathing and heartbeat on the experiment
The in vitro method is to directly take out the intestinal tissue, and use a constant temperature device to keep the tissue active in an environment containing oxygen and nutrient solution. Under this condition, the tissue can survive in vitro for 3 to 4 hours (or even longer, such as overnight ), so as to obtain a relatively accurate motion waveform. This method avoids the interference of breathing and heartbeat on the experiment, but the accuracy still needs to be improved

Method used

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  • In-vitro detection system for intestinal migration compound motion and application thereof
  • In-vitro detection system for intestinal migration compound motion and application thereof
  • In-vitro detection system for intestinal migration compound motion and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1 Design and construction of in vitro detection system

[0050] This embodiment provides an in vitro detection system for intestinal migratory compound movement, the schematic diagram is as follows figure 1 As shown, the in vitro detection system includes a sample fixing device, a constant temperature heater, a liquid storage tank, a peristaltic pump and a waste liquid collection tank;

[0051] The sample fixing device is located on the constant temperature heater;

[0052] The sample fixing device, liquid storage cylinder and peristaltic pump are connected through pipelines;

[0053] The peristaltic pump is used to pump the liquid in the liquid storage cylinder into the sample fixing device through the liquid inlet pipe, and the liquid in the sample fixing device flows into the waste liquid collection cylinder through the liquid outlet pipe;

[0054] Such as Figure 2A As shown, the sample fixing device is a black silicone rubber container;

[0055] The ...

Embodiment 2

[0062] Example 2 In Vitro Detection of Intestinal Migratory Compound Movement

[0063] In this example, the in vitro detection system of Example 1 is used for the in vitro detection of intestinal migratory compound movement, and the steps are as follows:

[0064] (1) Prepare Krebs buffer (120.9mM NaCl, 5.9mM KCl, 1.2mM MgCl 2 , 2.5mM CaCl 2 , 1.2mM NaH 2 PO 4 , 14.4mM NaHCO 3 and 11.5mM glucose), after loading the liquid storage tank, open the oxygen supply device and pass 95% O to the liquid storage tank 2 and 5% CO 2 , and at the same time, place the liquid storage tank in a water bath to maintain 50°C;

[0065] (2) Place the black silicone rubber sample fixing device used for fixing tissue on the constant temperature heater, adjust the temperature to 37°C, turn on the peristaltic pump, and let the Krebs buffer solution in the liquid storage tank pass through the inlet tube from the inlet of the sample fixing device. The liquid hole is pumped into the sample fixing de...

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Abstract

The invention provides an in-vitro detection system for intestinal migration compound motion and application thereof. The in-vitro detection system comprises a sample fixing device, a constant-temperature heater, a liquid storage cylinder, a peristaltic pump and a waste liquid collection cylinder, wherein the sample fixing device is positioned on the constant-temperature heater; the sample fixing device, the liquid storage cylinder and the peristaltic pump are connected through pipelines; the peristaltic pump is used for pumping liquid in the liquid storage cylinder into the sample fixing device through the liquid inlet pipe, and the liquid in the sample fixing device flows into the waste liquid collecting cylinder through the liquid outlet pipe. Tissues are kept active in an in-vitro state by adopting a mode of circularly heating and pouring a buffer solution, the in-vivo motion state of intestinal tracts is simulated to the greatest extent, and in-vitro detection of intestinal migration composite motion is realized by utilizing a video recording mode.

Description

technical field [0001] The invention belongs to the technical field of neuroscience, and relates to an in vitro detection system of intestinal migratory compound movement and its application. Background technique [0002] As we all know, the gastrointestinal tract is the only way to absorb nutrients, and it is also an important organ that controls the balance of the body's internal environment. The gastrointestinal tract, on the one hand absorbs energy and nutrients from ingested food for use by the body, and on the other hand protects the body from toxins, pathogens, etc., and the coordination of these complex basic activities is largely dependent on the local nervous system To complete, the enteric nervous system (Enteric nervous system, ENS). ENS is the largest peripheral nervous system. It is a reticular nerve plexus structure composed of neurons and glial cells located in the intestinal wall, mainly including submucous plexus and myenteric plexus. The larger myenteric...

Claims

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Application Information

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IPC IPC(8): G01N33/483B01L1/00
CPCG01N33/4833B01L1/00
Inventor 李志玲湛琴琴詹阳
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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