In-vitro detection system for intestinal migration compound motion and application thereof

Abstract

The invention provides an in-vitro detection system for intestinal migration compound motion and application thereof. The in-vitro detection system comprises a sample fixing device, a constant-temperature heater, a liquid storage cylinder, a peristaltic pump and a waste liquid collection cylinder, wherein the sample fixing device is positioned on the constant-temperature heater; the sample fixing device, the liquid storage cylinder and the peristaltic pump are connected through pipelines; the peristaltic pump is used for pumping liquid in the liquid storage cylinder into the sample fixing device through the liquid inlet pipe, and the liquid in the sample fixing device flows into the waste liquid collecting cylinder through the liquid outlet pipe. Tissues are kept active in an in-vitro state by adopting a mode of circularly heating and pouring a buffer solution, the in-vivo motion state of intestinal tracts is simulated to the greatest extent, and in-vitro detection of intestinal migration composite motion is realized by utilizing a video recording mode.

Description

technical field [0001] The invention belongs to the technical field of neuroscience, and relates to an in vitro detection system of intestinal migratory compound movement and its application. Background technique [0002] As we all know, the gastrointestinal tract is the only way to absorb nutrients, and it is also an important organ that controls the balance of the body's internal environment. The gastrointestinal tract, on the one hand absorbs energy and nutrients from ingested food for use by the body, and on the other hand protects the body from toxins, pathogens, etc., and the coordination of these complex basic activities is largely dependent on the local nervous system To complete, the enteric nervous system (Enteric nervous system, ENS). ENS is the largest peripheral nervous system. It is a reticular nerve plexus structure composed of neurons and glial cells located in the intestinal wall, mainly including submucous plexus and myenteric plexus. The larger myenteric...

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Problems solved by technology

In the in vivo method, the intestinal tract is usually pulled out from the body and fixed, and the reagent is directly added dropwise to the serosa layer of the intestinal tract, and the body temperature and nutrient supply of the intestinal tract are maintained by using the animal's own blood circulation. Exposure may cause tissue damage and prevent normal movement; there is also a method of in vivo testing using water-isolated and constant-temperature heating of the groove containing intestinal tissue. The disadvantage is that it is inconvenient to operate, and the fixed length, height and position of the small intestine in the groove are difficult to adjust , cannot effectively remove the interference of breathing and heartbeat on the experiment

The in vitro method is to directly take out the intestinal tissue, and use a constant temperature device to keep the tissue active in an environment containing oxygen and nutrient solution. Under this condition, the tissue can survive in vitro for 3 to 4 hours (or even longer, such as overnight ), so as to obtain a relatively accurate motion waveform. This method avoids the interference of breathing and heartbeat on the experiment, but the accuracy still needs to be improved

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