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Buffer solution for storing superparamagnetic particles and protein connecting substances thereof and preparation method of buffer solution

A buffer, superparamagnetic technology, applied in the field of protein preservation, can solve the problems of difficult magnetic particles, mixing, repeatability deviation, etc.

Active Publication Date: 2021-04-27
SOPHONIX CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for reaction consumables with special shapes (such as V-shaped bottom reaction cups), this method is not easy to mix the magnetic particles, which will cause large repeatability deviations

Method used

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  • Buffer solution for storing superparamagnetic particles and protein connecting substances thereof and preparation method of buffer solution
  • Buffer solution for storing superparamagnetic particles and protein connecting substances thereof and preparation method of buffer solution
  • Buffer solution for storing superparamagnetic particles and protein connecting substances thereof and preparation method of buffer solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A buffer for the preservation of superparamagnetic microparticles and their protein connectors, comprising the following steps:

[0037] (1) Weigh 500ml of purified water, add 5.6g of disodium hydrogen phosphate dodecahydrate, 0.55g of sodium dihydrogen phosphate, 9g of sodium chloride, 30g of bovine serum albumin, 70g of sucrose and 3ml of Tween-20; stir to dissolve ; Make base buffer;

[0038] (2) Add 0.3g of xanthan gum, 0.5g of sodium alginate, and 15g of gelatin to the basic buffer in sequence, stir and heat at 35°C for 12h, adjust the pH value of the solution to 7.0, add purified water to 1L, and use 0.22μm Filter through a filter membrane to obtain the buffer solution.

Embodiment 2

[0040] A buffer for the preservation of superparamagnetic microparticles and their protein connectors, comprising the following steps:

[0041] (1) Weigh 500ml of purified water, add 5.9g of disodium hydrogen phosphate dodecahydrate, 0.60g of sodium dihydrogen phosphate, 5g of sodium chloride, 10g of bovine serum albumin, 135g of sucrose and 1ml of Tween-60; stir to dissolve ; Make base buffer;

[0042] (2) Add 0.5g of xanthan gum, 0.5g of sodium alginate, and 5g of gelatin to the basic buffer in turn, stir and heat at 42°C for 16h, adjust the pH value of the solution to 7.6, add purified water to 1L, and use 0.2μm Filter through a filter membrane to obtain the buffer solution.

Embodiment 3

[0044] A buffer for the preservation of superparamagnetic microparticles and their protein connectors, comprising the following steps:

[0045] (1) Take 500ml of purified water, add 4g of disodium hydrogen phosphate dodecahydrate, 1g of sodium dihydrogen phosphate, 5g of sodium chloride, 1g of bovine serum albumin, 150g of sucrose and 1ml of Tween-80; stir to dissolve; into a basic buffer;

[0046] (2) Add 0.1g of xanthan gum, 0.1g of sodium alginate, and 1g of gelatin to the basic buffer in turn, stir and heat at 30°C for 16h, adjust the pH value of the solution to 7.2, add purified water to 1L, and use 0.5μm Filter through a membrane filter to obtain the buffer solution.

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PUM

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Abstract

The invention belongs to the technical field of protein preservation, and relates to a buffer solution for storing superparamagnetic particles and protein connecting substances thereof and a preparation method of the buffer solution. The buffer solution for storing the superparamagnetic particles and the protein connectors thereof comprises the following components of: disodium hydrogen phosphate dodecahydrate, sodium dihydrogen phosphate, sodium chloride, bovine serum albumin, cane sugar, xanthan gum, sodium alginate and gelatin. According to the buffer agent provided by the invention, micron-sized superparamagnetic particles and connecting substances of the superparamagnetic particles and specific proteins can be stored for a long time, so that the micron-sized superparamagnetic particles and the connecting substances do not lose efficacy due to physical effects (such as sedimentation and hardening); the buffer solution is a special buffer solution, and is used for long-term storage of superparamagnetic particles and protein connecting substances of the superparamagnetic particles.

Description

technical field [0001] The invention belongs to the technical field of protein preservation, and in particular relates to a buffer and a preparation method for the preservation of superparamagnetic particles and their protein junctions. Background technique [0002] Magnetic particle chemiluminescence is an advanced immunological detection method that can detect specific protein components in blood in vitro. This method can effectively assist doctors in assisting diagnosis of patients' conditions. Protein conjugation to micron-sized superparamagnetic particles plays an important role in this methodology. [0003] However, the protein conjugates of superparamagnetic particles are very easy to settle and because of their poor hydrophilicity, they will gradually compact into a sheet or block structure after settling. The hardened magnetic particle-protein linker is very unstable, which will cause the overall failure of the reaction system and lead to product failure. At pres...

Claims

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Application Information

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IPC IPC(8): G01N33/531G01N33/543
CPCG01N33/531G01N33/54326
Inventor 王法龙孙家刘聪李博飞
Owner SOPHONIX CO LTD
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