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Application of baicalein compound to preparation of medicine for promoting differentiation of neural stem cells

A technology of neural stem cells and baicalein, applied in the field of regenerative medicine, can solve the problems that baicalein has not yet been discovered

Pending Publication Date: 2021-05-07
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current reports about baicalein on nerve injury are mostly researched from the aspects of reducing apoptosis, cell protection, and pain relief. No reports have been found whether baicalein can directly promote the differentiation of neural stem cells.

Method used

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  • Application of baicalein compound to preparation of medicine for promoting differentiation of neural stem cells
  • Application of baicalein compound to preparation of medicine for promoting differentiation of neural stem cells
  • Application of baicalein compound to preparation of medicine for promoting differentiation of neural stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Evaluation of the effect of baicalein on the differentiation of neural stem cells in vitro

[0070] 1.1 Neural stem cell differentiation culture

[0071]Before the experiment, different concentrations of baicalein were encapsulated in liposomes, and the control group was blank liposomes without baicalein. At the beginning of the experiment, the cultured rat primary neural stem cells were centrifuged (300rcf, 5min), digested, and centrifuged again, and the cells were resuspended in a high-glucose medium containing 10% fetal bovine serum, added to a 96-well plate, and added to a 96-well plate. Well about 10,000 cells, overnight in the cell culture incubator. After the cells adhered to the wall the next day, the cell culture medium was replaced with differentiation medium containing different baicalein concentrations, and the medium was changed every 3 days during the culture process.

[0072] 1.2 Identification of the effect of baicalein on the differentiation of neural...

Embodiment 2

[0076] Evaluation of the therapeutic effect of baicalein on total spinal cord transection injury

[0077] From the results in vitro, baicalein can promote the differentiation of neural stem cells into neurons. In order to explore whether baicalein can promote endogenous neurogenesis after spinal cord transection injury, so as to achieve the effect of injury repair. The inventors of this case constructed a rat model of spinal cord injury with complete transection, and implanted collagen materials loaded with baicalein into the injury after transection, and the control group was collagen materials without baicalein. One week after the injury, the experimental rats were perfused, samples were taken, and tissue sections were stained. And the recovery of motor function of the rats was recorded every week until 8 weeks. The following is the specific implementation plan:

[0078] 2.1 Establishment of spinal cord total transection injury model

[0079] The experimental animals wer...

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Abstract

The invention discloses an application of a baicalein compound to preparation of a medicine for promoting differentiation of neural stem cells. The application comprises an application of the baicalein compound to preparation of a medicine for promoting neural stem cells to differentiate into neurons, a medicine for inhibiting the neural stem cells from differentiating into astrocytes or a medicine for relieving and / or treating nerve injury repair. The medicine at least can promote the neural stem cells to differentiate towards neurons and inhibit the neural stem cells from differentiating towards astrocytes, so that nerve injury repair is realized. According to the application disclosed by the invention, the effect of baicalein for promoting the neural stem cells to differentiate towards the neurons rather than the astrocytes is found for the first time, the baicalein has a more direct and effective effect on repairing nerve injury, and the effect of the baicalein serving as a nerve treatment medicine is directly affirmed. Besides, a new method and approach are provided for nerve injury repair treatment.

Description

technical field [0001] The present invention relates to the use of a baicalein compound, in particular to a baicalein compound, its isomer and pharmaceutically acceptable derivatives thereof and a pharmaceutical composition containing the compound in the preparation of neural stem cell differentiation-promoting The use in medicine belongs to the technical field of regenerative medicine. Background technique [0002] Nerve cells cannot regenerate after injury, which often leads to degeneration and paralysis of corresponding functions. The research on nerve repair damage has always been a difficult point in regenerative medicine. After nerve injury occurs, it will cause a series of neuron death, axon loss, and even blood vessel damage, which will cause a series of inflammation and further deteriorate the damage microenvironment, making nerve differentiation and regeneration very difficult. In recent years, nerve injury regeneration and repair technology has been continuously ...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P25/28A61P25/00A61P29/00
CPCA61K31/352A61P25/28A61P25/00A61P29/00
Inventor 陈艳艳钱琳戴建武
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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