Spectral measurement system and method for biological delayed luminescence

Abstract

The invention discloses a spectral measurement system and method for biological delayed luminescence, and belongs to the technical field of spectral measurement. The system comprises an exciting light source for receiving a pulse signal, controlling the exciting light source to emit a light source signal with a preset wavelength according to the pulse signal, and coupling the light source signal to a photon collection system by using an optical fiber, a photon collector irradiating the coupled light source signal to a target organism, collecting DL photons excited by the coupled light source signal of the target organism, collecting the DL photons by using a special optical fiber bundle, and outputting a spectral image after splitting the collected DL photons, and a controller used for completing spectral measurement of biological delayed luminescence according to the spectral image. Compared with the existing filter wheel type DL spectrum measurement system, the DL photon fine spectrum delay curve can be obtained, and the measurement speed is high.

Description

technical field [0001] The present invention relates to the technical field of spectroscopic measurement, and more specifically, to a spectroscopic measurement system and method for delayed luminescence of organisms. Background technique [0002] Delayed Luminescence (DL for short) is weak light in the wavelength range of 400nm to 800nm ​​emitted by biomolecules after the light beam stimulates the organism. The general delay time is on the order of ms. As time decays, the change curve of photon number with time shows Hyperbolic rather than exponential decay like fluorescence. This kind of bioluminescence is called biophoton, but the process of biophoton emission has not been fully explained yet. In short, various molecular atoms in the organism participate in the interaction with these photons, so they have a wide molecular spectrum. Therefore, changes in basic biological processes such as metabolism and gene expression will cause related changes in biophoton emission, whic...

AI Technical Summary

Problems solved by technology

Most of the others basically use this filter wheel to solve DL spectrum acquisition, and have improved the control of PMT, but they still need to switch the filter wheel when collecting spectra, and use grating spectrometers to collect spectra. However, Can only collect very strong fluorescence spectra in the first us

[0008] In order to collect more photon filters, the bandwidth of the general filter is 30-50nm, so the fine structure of the DL emission spectrum cannot be obtained. From the perspective of the molecular spectrum, a resolution of 1-3nm is required to identify the molecular spectrum

[0009] The traditional grating spectrometer combined with low-noise and high-sensitivity PMT can collect the fluorescent part of bioluminescence, that is, measure the fluorescent part with high luminous intensity within a few us immediately after excitation, but cannot measure the delayed luminescence later

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