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Centrifugal separation device for cell separation

A centrifugal separation and cell technology, applied in the field of cell separation, can solve the problems of low degree of automation and prolonged separation cycle

Active Publication Date: 2021-05-28
JILIN UNIV FIRST HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] After a single separation in the existing cell centrifugal separation device, the staff needs to manually extract the precipitated structure and transfer the homogenate for the next step of centrifugal separation. The degree of automation is low, resulting in prolonged separation cycle

Method used

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  • Centrifugal separation device for cell separation
  • Centrifugal separation device for cell separation
  • Centrifugal separation device for cell separation

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Embodiment Construction

[0025] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative work fall within the protection scope of the present invention. The present invention will be further described below in conjunction with the embodiments.

[0026] see figure 1 , image 3 , Figure 4 with Figure 5 , in an embodiment of the present invention, a centrifugal separation device for cell separation, including a machine base 1, a controller 2 and a cell homogenization box 4 are fixedly installed on the machine base 1, and one end of the cell homogenization box 4 rotates A centrifuge bucket 3 is installed, and a plurali...

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Abstract

The invention relates to the technical field of cell separation, in particular to a centrifugal separation device for cell separation. The device comprises a base, a controller and a cell homogenizing tank are fixedly mounted on the base, a centrifugal barrel is rotatably mounted at one end of the cell homogenizing tank, a centrifugal cavity and a precipitation cavity are respectively arranged in the centrifugal barrel from top to bottom, a stepping motor used for driving the centrifugal barrel is fixedly installed on one side of the cell homogenizing tank, the output end of the stepping motor is connected with the centrifugal barrel through a transmission part, a transposition disc is rotationally installed on the base, a plurality of material storage containers are detachably installed on the transposition disc in the circumferential direction, a material receiving disc is arranged over the transposition disc, and plurality of falling grooves are formed in the material receiving disc in the circumferential direction. By arranging a linkage structure, the automation degree of the whole process is improved, and the period of the whole separation procedure is shortened.

Description

technical field [0001] The invention relates to the technical field of cell separation, in particular to a centrifugal separation device for cell separation. Background technique [0002] Cell separation techniques include centrifugation, flow cytometry, and cell electrophoresis. Centrifugation is the basic method for studying nuclei, mitochondria, Golgi bodies, lysosomes, and microbodies, as well as various macromolecules. A technique for rapid quantitative analysis and sorting, cell electrophoresis refers to the fact that the cell surface has a net positive or negative charge at a certain pH value, and can swim under the action of an external electric field. [0003] After a single separation, the existing cell centrifugation device requires staff to manually extract the precipitated structure and transfer the homogenate for the next step of centrifugation. The degree of automation is low and the separation cycle is prolonged. Contents of the invention [0004] The obje...

Claims

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Application Information

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IPC IPC(8): B04B5/00B04B7/08B04B11/04B04B11/05B04B9/02
CPCB04B5/00B04B7/08B04B11/04B04B11/05B04B9/02
Inventor 于得海
Owner JILIN UNIV FIRST HOSPITAL
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